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@INPROCEEDINGS{Metzner:905566,
      author       = {Metzner, Ralf and Chlubek, Antonia and Bühler, Jonas and
                      Pflugfelder, Daniel and Schurr, Ulrich and Huber, Gregor and
                      Koller, Robert and Jahnke, Siegfried},
      title        = {{C}arbon dynamics in nodulated pea root systems: 3{D}
                      imaging andquantification with short lived isotopes},
      reportid     = {FZJ-2022-00807},
      year         = {2021},
      abstract     = {Legumes associate with root colonizing rhizobia that
                      provide atmospheric nitrogen to its plant host in exchange
                      for recently fixed carbon. While much of the nodulation
                      process and its regulation is now understood, there is a
                      lack in understanding how plants modulate carbon allocation
                      to a nodulated root system as a dynamic response to abiotic
                      stimuli. One reason is that most approaches are based on
                      destructive sampling, making investigation of localized
                      carbon allocation dynamics in the root system difficult. We
                      employed non-invasive Positron Emission Tomography (PET) to
                      follow the allocation of leaf-supplied 11C tracer towards
                      individual nodules in a three-dimensional (3D) root system
                      of pea (Pisum sativum). Nitrate was applied to the root
                      system to rapidly shut down biological nitrogen fixation and
                      follow the effect on carbon allocation dynamics. This
                      treatment lead to a reduction of 11C tracer allocation to
                      nodules by $40\%$ - $47\%$ in 5 treated plants within 42h
                      while the change in control plants was less than $11\%.$ Our
                      study demonstrates the strength of using 11C tracers in a
                      PET approach for non-invasive quantification of dynamic
                      carbon allocation in growing plants over several days. A
                      major advantage of the approach is the possibility to
                      investigate carbon dynamics in small regions of interest in
                      a 3D system such as nodules in comparison to whole plant
                      development.},
      month         = {Jun},
      date          = {2021-06-28},
      organization  = {Plant Biology Europe 2021, Turin
                       (Italy), 28 Jun 2021 - 1 Jul 2021},
      subtyp        = {After Call},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/905566},
}