000905938 001__ 905938 000905938 005__ 20230123110555.0 000905938 0247_ $$2doi$$a10.1016/j.molp.2022.01.003 000905938 0247_ $$2ISSN$$a1674-2052 000905938 0247_ $$2ISSN$$a1752-9859 000905938 0247_ $$2ISSN$$a1752-9867 000905938 0247_ $$2Handle$$a2128/30935 000905938 0247_ $$2altmetric$$aaltmetric:120648354 000905938 0247_ $$2pmid$$apmid:35026436 000905938 0247_ $$2WOS$$aWOS:000767562700006 000905938 037__ $$aFZJ-2022-01120 000905938 082__ $$a580 000905938 1001_ $$0P:(DE-HGF)0$$aHoopes, Genevieve$$b0 000905938 245__ $$aPhased, chromosome-scale genome assemblies of tetraploid potato reveals a complex genome, transcriptome, and predicted proteome landscape underpinning genetic diversity 000905938 260__ $$aOxford$$bOxford Univ. Press$$c2022 000905938 3367_ $$2DRIVER$$aarticle 000905938 3367_ $$2DataCite$$aOutput Types/Journal article 000905938 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1648472189_16318 000905938 3367_ $$2BibTeX$$aARTICLE 000905938 3367_ $$2ORCID$$aJOURNAL_ARTICLE 000905938 3367_ $$00$$2EndNote$$aJournal Article 000905938 520__ $$aCultivated potato is a clonally propagated autotetraploid species with a highly heterogeneous genome. Phased assemblies of six cultivars including two chromosome-scale phased genome assemblies revealed extensive allelic diversity, including altered coding and transcript sequences, preferential allele expression, and structural variation that collectively result in a highly complex transcriptome and predicted proteome, which are distributed across the homologous chromosomes. Wild species contribute to the extensive allelic diversity in tetraploid cultivars, demonstrating ancestral introgressions predating modern breeding efforts. As a clonally propagated autotetraploid that undergoes limited meiosis, dysfunctional and deleterious alleles are not purged in tetraploid potato. Nearly a quarter of the loci bore mutations are predicted to have a high negative impact on protein function, complicating breeder’s efforts to reduce genetic load. The StCDF1 locus controls maturity, and analysis of six tetraploid genomes revealed that 12 allelic variants of StCDF1 are correlated with maturity in a dosage-dependent manner. Knowledge of the complexity of the tetraploid potato genome with its rampant structural variation and embedded deleterious and dysfunctional alleles will be key not only to implementing precision breeding of tetraploid cultivars but also to the construction of homozygous, diploid potato germplasm containing favorable alleles to capitalize on heterosis in F1 hybrids. 000905938 536__ $$0G:(DE-HGF)POF4-2171$$a2171 - Biological and environmental resources for sustainable use (POF4-217)$$cPOF4-217$$fPOF IV$$x0 000905938 588__ $$aDataset connected to CrossRef, Journals: juser.fz-juelich.de 000905938 7001_ $$0P:(DE-HGF)0$$aMeng, Xiaoxi$$b1 000905938 7001_ $$0P:(DE-HGF)0$$aHamilton, John P.$$b2 000905938 7001_ $$0P:(DE-HGF)0$$aAchakkagari, Sai Reddy$$b3 000905938 7001_ $$0P:(DE-HGF)0$$ade Alves Freitas Guesdes, Fernanda$$b4 000905938 7001_ $$0P:(DE-Juel1)162335$$aBolger, Marie E.$$b5 000905938 7001_ $$0P:(DE-HGF)0$$aCoombs, Joseph J.$$b6 000905938 7001_ $$0P:(DE-HGF)0$$aEsselink, Danny$$b7 000905938 7001_ $$0P:(DE-HGF)0$$aKaiser, Natalie R.$$b8 000905938 7001_ $$0P:(DE-HGF)0$$aKodde, Linda$$b9 000905938 7001_ $$0P:(DE-HGF)0$$aKyriakidou, Maria$$b10 000905938 7001_ $$0P:(DE-HGF)0$$aLavrijssen, Brian$$b11 000905938 7001_ $$0P:(DE-HGF)0$$avan Lieshout, Natascha$$b12 000905938 7001_ $$0P:(DE-HGF)0$$aShereda, Rachel$$b13 000905938 7001_ $$0P:(DE-HGF)0$$aTuttle, Heather K.$$b14 000905938 7001_ $$0P:(DE-HGF)0$$aVaillancourt, Brieanne$$b15 000905938 7001_ $$0P:(DE-HGF)0$$aWood, Joshua C.$$b16 000905938 7001_ $$0P:(DE-HGF)0$$ade Boer, Jan M.$$b17 000905938 7001_ $$0P:(DE-HGF)0$$aBornowski, Nolan$$b18 000905938 7001_ $$0P:(DE-HGF)0$$aBourke, Peter$$b19 000905938 7001_ $$0P:(DE-HGF)0$$aDouches, David$$b20 000905938 7001_ $$0P:(DE-HGF)0$$avan Eck, Herman J.$$b21 000905938 7001_ $$0P:(DE-HGF)0$$aEllis, Dave$$b22 000905938 7001_ $$0P:(DE-HGF)0$$aFeldman, Max J.$$b23 000905938 7001_ $$0P:(DE-HGF)0$$aGardner, Kyle M.$$b24 000905938 7001_ $$0P:(DE-HGF)0$$aHopman, Johannes C. P.$$b25 000905938 7001_ $$0P:(DE-HGF)0$$aJiang, Jiming$$b26 000905938 7001_ $$0P:(DE-HGF)0$$aDe Jong, Walter S.$$b27 000905938 7001_ $$0P:(DE-HGF)0$$aKuhl, Joseph C.$$b28 000905938 7001_ $$0P:(DE-HGF)0$$aNovy, Richard G.$$b29 000905938 7001_ $$0P:(DE-HGF)0$$aOome, Stan$$b30 000905938 7001_ $$0P:(DE-HGF)0$$aSathuvalli, Vidyasagar$$b31 000905938 7001_ $$0P:(DE-HGF)0$$aTan, Ek Han$$b32 000905938 7001_ $$0P:(DE-HGF)0$$aUrsum, Remco A.$$b33 000905938 7001_ $$0P:(DE-HGF)0$$aVales, M. Isabel$$b34 000905938 7001_ $$0P:(DE-HGF)0$$aVining, Kelly$$b35 000905938 7001_ $$0P:(DE-HGF)0$$aVisser, Richard G. F.$$b36 000905938 7001_ $$0P:(DE-HGF)0$$aVossen, Jack$$b37 000905938 7001_ $$0P:(DE-HGF)0$$aYencho, G. Craig$$b38 000905938 7001_ $$0P:(DE-HGF)0$$aAnglin, Noelle L.$$b39 000905938 7001_ $$0P:(DE-HGF)0$$aBachem, Christian W. B.$$b40 000905938 7001_ $$0P:(DE-HGF)0$$aEndelman, Jeffrey B.$$b41 000905938 7001_ $$0P:(DE-HGF)0$$aShannon, Laura M.$$b42 000905938 7001_ $$0P:(DE-HGF)0$$aStrömvik, Martina V.$$b43 000905938 7001_ $$0P:(DE-HGF)0$$aTai, Helen H.$$b44 000905938 7001_ $$0P:(DE-Juel1)145719$$aUsadel, Björn$$b45 000905938 7001_ $$0P:(DE-HGF)0$$aBuell, C. Robin$$b46 000905938 7001_ $$00000-0002-4368-8058$$aFinkers, Richard$$b47$$eCorresponding author 000905938 773__ $$0PERI:(DE-600)2393618-6$$a10.1016/j.molp.2022.01.003$$gp. 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