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@ARTICLE{Metzner:906584,
      author       = {Metzner, Ralf and Chlubek, Antonia and Bühler, Jonas and
                      Pflugfelder, Daniel and Schurr, Ulrich and Huber, Gregor and
                      Koller, Robert and Jahnke, Siegfried},
      title        = {{I}n {V}ivo {I}maging and {Q}uantification of {C}arbon
                      {T}racer {D}ynamics in {N}odulated {R}oot {S}ystems of {P}ea
                      {P}lants},
      journal      = {Plants},
      volume       = {11},
      number       = {5},
      issn         = {2223-7747},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2022-01535},
      pages        = {632 -},
      year         = {2022},
      abstract     = {Legumes associate with root colonizing rhizobia that
                      provide fixed nitrogen to its plant host in exchange for
                      recently fixed carbon. There is a lack of understanding of
                      how individual plants modulate carbon allocation to a
                      nodulated root system as a dynamic response to abiotic
                      stimuli. One reason is that most approaches are based on
                      destructive sampling, making quantification of localised
                      carbon allocation dynamics in the root system difficult. We
                      established an experimental workflow for routinely using
                      non-invasive Positron Emission Tomography (PET) to follow
                      the allocation of leaf-supplied 11C tracer towards
                      individual nodules in a three-dimensional (3D) root system
                      of pea (Pisum sativum). Nitrate was used for triggering a
                      reduction of biological nitrogen fixation (BNF), which was
                      expected to rapidly affect carbon allocation dynamics in the
                      root-nodule system. The nitrate treatment led to a decrease
                      in 11C tracer allocation to nodules by $40\%$ to $47\%$ in 5
                      treated plants while the variation in control plants was
                      less than $11\%.$ The established experimental pipeline
                      enabled for the first time that several plants could
                      consistently be labelled and measured using 11C tracers in a
                      PET approach to quantify C-allocation to individual nodules
                      following a BNF reduction. Our study demonstrates the
                      strength of using 11C tracers in a PET approach for
                      non-invasive quantification of dynamic carbon allocation in
                      several growing plants over several days. A major advantage
                      of the approach is the possibility to investigate carbon
                      dynamics in small regions of interest in a 3D system such as
                      nodules in comparison to whole plant development.},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {35270102},
      UT           = {WOS:000768174800001},
      doi          = {10.3390/plants11050632},
      url          = {https://juser.fz-juelich.de/record/906584},
}