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@ARTICLE{Metzner:906584,
author = {Metzner, Ralf and Chlubek, Antonia and Bühler, Jonas and
Pflugfelder, Daniel and Schurr, Ulrich and Huber, Gregor and
Koller, Robert and Jahnke, Siegfried},
title = {{I}n {V}ivo {I}maging and {Q}uantification of {C}arbon
{T}racer {D}ynamics in {N}odulated {R}oot {S}ystems of {P}ea
{P}lants},
journal = {Plants},
volume = {11},
number = {5},
issn = {2223-7747},
address = {Basel},
publisher = {MDPI},
reportid = {FZJ-2022-01535},
pages = {632 -},
year = {2022},
abstract = {Legumes associate with root colonizing rhizobia that
provide fixed nitrogen to its plant host in exchange for
recently fixed carbon. There is a lack of understanding of
how individual plants modulate carbon allocation to a
nodulated root system as a dynamic response to abiotic
stimuli. One reason is that most approaches are based on
destructive sampling, making quantification of localised
carbon allocation dynamics in the root system difficult. We
established an experimental workflow for routinely using
non-invasive Positron Emission Tomography (PET) to follow
the allocation of leaf-supplied 11C tracer towards
individual nodules in a three-dimensional (3D) root system
of pea (Pisum sativum). Nitrate was used for triggering a
reduction of biological nitrogen fixation (BNF), which was
expected to rapidly affect carbon allocation dynamics in the
root-nodule system. The nitrate treatment led to a decrease
in 11C tracer allocation to nodules by $40\%$ to $47\%$ in 5
treated plants while the variation in control plants was
less than $11\%.$ The established experimental pipeline
enabled for the first time that several plants could
consistently be labelled and measured using 11C tracers in a
PET approach to quantify C-allocation to individual nodules
following a BNF reduction. Our study demonstrates the
strength of using 11C tracers in a PET approach for
non-invasive quantification of dynamic carbon allocation in
several growing plants over several days. A major advantage
of the approach is the possibility to investigate carbon
dynamics in small regions of interest in a 3D system such as
nodules in comparison to whole plant development.},
cin = {IBG-2},
ddc = {580},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {2171 - Biological and environmental resources for
sustainable use (POF4-217)},
pid = {G:(DE-HGF)POF4-2171},
typ = {PUB:(DE-HGF)16},
pubmed = {35270102},
UT = {WOS:000768174800001},
doi = {10.3390/plants11050632},
url = {https://juser.fz-juelich.de/record/906584},
}