TY  - JOUR
AU  - Cheung, Giselle
AU  - Bataveljic, Danijela
AU  - Visser, Josien
AU  - Kumar, Naresh
AU  - Moulard, Julien
AU  - Dallérac, Glenn
AU  - Mozheiko, Daria
AU  - Rollenhagen, Astrid
AU  - Ezan, Pascal
AU  - Mongin, Cédric
AU  - Chever, Oana
AU  - Bemelmans, Alexis-Pierre
AU  - Lübke, Joachim
AU  - Leray, Isabelle
AU  - Rouach, Nathalie
TI  - Physiological synaptic activity and recognition memory require astroglial glutamine
JO  - Nature Communications
VL  - 13
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - FZJ-2022-01660
SP  - 753
PY  - 2022
AB  - Presynaptic glutamate replenishment is fundamental to brain function. In high activity regimes, such as epileptic episodes, this process is thought to rely on the glutamate-glutamine cycle between neurons and astrocytes. However the presence of an astroglial glutamine supply, as well as its functional relevance in vivo in the healthy brain remain controversial, partly due to a lack of tools that can directly examine glutamine transfer. Here, we generated a fluorescent probe that tracks glutamine in live cells, which provides direct visual evidence of an activity-dependent glutamine supply from astroglial networks to presynaptic structures under physiological conditions. This mobilization is mediated by connexin43, an astroglial protein with both gap-junction and hemichannel functions, and is essential for synaptic transmission and object recognition memory. Our findings uncover an indispensable recruitment of astroglial glutamine in physiological synaptic activity and memory via an unconventional pathway, thus providing an astrocyte basis for cognitive processes.
LB  - PUB:(DE-HGF)16
C6  - 35136061
UR  - <Go to ISI:>//WOS:000757297200017
DO  - DOI:10.1038/s41467-022-28331-7
UR  - https://juser.fz-juelich.de/record/906738
ER  -