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@ARTICLE{Kugele:906750,
author = {Kugele, Anandi and Uzun, Buket and Müller, Lena and
Schott-Verdugo, Stephan and Gohlke, Holger and Groth, Georg
and Drescher, Malte},
title = {{M}apping the helix arrangement of the reconstituted {ETR}1
ethylene receptor transmembrane domain by {EPR}
spectroscopy},
journal = {RSC Advances},
volume = {12},
issn = {2046-2069},
address = {London},
publisher = {RSC Publishing},
reportid = {FZJ-2022-01668},
pages = {7352 - 7356},
year = {2022},
abstract = {The plant ethylene receptor ETR1 is a key player in the
perception of the phytohormone and subsequent downstream
ethylene signal transmission, crucial for processes such as
ripening, senescence and abscission. However, to date, there
is sparse structural knowledge about the transmembrane
sensor domain (TMD) of ETR1 that is responsible for the
binding of the plant hormone and initiates the downstream
signal transmission. Sequence information and ab initio
modelling suggest that the TMD consists of three
transmembrane helices. Here, we combined site-directed spin
labelling with electron paramagnetic resonance spectroscopy
and obtained distance restraints for liposome-reconstituted
$ETR1_TMD$ on the orientation and arrangement of the
transmembrane helices. We used these data to scrutinize
different computational structure predictions of the TMD.},
cin = {JSC / NIC / IBI-7 / IBG-4},
ddc = {540},
cid = {I:(DE-Juel1)JSC-20090406 / I:(DE-Juel1)NIC-20090406 /
I:(DE-Juel1)IBI-7-20200312 / I:(DE-Juel1)IBG-4-20200403},
pnm = {5111 - Domain-Specific Simulation $\&$ Data Life Cycle Labs
(SDLs) and Research Groups (POF4-511) / 2171 - Biological
and environmental resources for sustainable use (POF4-217) /
2172 - Utilization of renewable carbon and energy sources
and engineering of ecosystem functions (POF4-217) /
Forschergruppe Gohlke $(hkf7_20200501)$ / 5241 - Molecular
Information Processing in Cellular Systems (POF4-524)},
pid = {G:(DE-HGF)POF4-5111 / G:(DE-HGF)POF4-2171 /
G:(DE-HGF)POF4-2172 / $G:(DE-Juel1)hkf7_20200501$ /
G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000764246300001},
doi = {10.1039/D2RA00604A},
url = {https://juser.fz-juelich.de/record/906750},
}