000906834 001__ 906834
000906834 005__ 20230123110608.0
000906834 0247_ $$2doi$$a10.3390/biom12030469
000906834 0247_ $$2Handle$$a2128/31018
000906834 0247_ $$2altmetric$$aaltmetric:125312699
000906834 0247_ $$2pmid$$apmid:35327661
000906834 0247_ $$2WOS$$aWOS:000775905700001
000906834 037__ $$aFZJ-2022-01728
000906834 082__ $$a570
000906834 1001_ $$0P:(DE-HGF)0$$aBraczynski, Anne K.$$b0
000906834 245__ $$aAlpha-Synuclein-Specific Naturally Occurring Antibodies Inhibit Aggregation In Vitro and In Vivo
000906834 260__ $$aBasel$$bMDPI$$c2022
000906834 3367_ $$2DRIVER$$aarticle
000906834 3367_ $$2DataCite$$aOutput Types/Journal article
000906834 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1649833513_23057
000906834 3367_ $$2BibTeX$$aARTICLE
000906834 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000906834 3367_ $$00$$2EndNote$$aJournal Article
000906834 520__ $$aParkinson’s disease (PD) is associated with motor and non-motor symptoms and characterized by aggregates of alpha-synuclein (αSyn). Naturally occurring antibodies (nAbs) are part of the innate immune system, produced without prior contact to their specific antigen, and polyreactive. The abundance of nAbs against αSyn is altered in patients with PD. In this work, we biophysically characterized nAbs against αSyn (nAbs-αSyn) and determined their biological effects. nAbs-αSyn were isolated from commercial intravenous immunoglobulins using column affinity purification. Biophysical properties were characterized using a battery of established in vitro assays. Biological effects were characterized in HEK293T cells transiently transfected with fluorescently tagged αSyn. Specific binding of nAbs-αSyn to monomeric αSyn was demonstrated by Dot blot, ELISA, and Surface Plasmon Resonance. nAbs-αSyn did not affect viability of HEK293T cells as reported by Cell Titer Blue and LDH Assays. nAbs-αSyn inhibited fibrillation of αSyn reported by the Thioflavin T aggregation assay. Altered fibril formation was confirmed with atomic force microscopy. In cells transfected with EGFP-tagged αSyn we observed reduced formation of aggresomes, perinuclear accumulations of αSyn aggregates. The results demonstrate that serum of healthy individuals contains nAbs that specifically bind αSyn and inhibit aggregation of αSyn in vitro. The addition of nAbs-αSyn to cultured cells affects intracellular αSyn aggregates. These findings help understanding the role of the innate immune systems for the pathogenesis of PD and suggest that systemic αSyn binding agents could potentially affect neuronal αSyn pathology.
000906834 536__ $$0G:(DE-HGF)POF4-5244$$a5244 - Information Processing in Neuronal Networks (POF4-524)$$cPOF4-524$$fPOF IV$$x0
000906834 588__ $$aDataset connected to DataCite
000906834 7001_ $$0P:(DE-Juel1)165152$$aSevenich, Marc$$b1$$ufzj
000906834 7001_ $$0P:(DE-Juel1)171922$$aGering, Ian$$b2
000906834 7001_ $$0P:(DE-Juel1)176456$$aKupreichyk, Tatsiana$$b3
000906834 7001_ $$0P:(DE-HGF)0$$aAgerschou, Emil D.$$b4
000906834 7001_ $$0P:(DE-HGF)0$$aKronimus, Yannick$$b5
000906834 7001_ $$00000-0002-5771-216X$$aHabib, Pardes$$b6
000906834 7001_ $$0P:(DE-Juel1)132023$$aStoldt, Matthias$$b7$$ufzj
000906834 7001_ $$0P:(DE-Juel1)132029$$aWillbold, Dieter$$b8
000906834 7001_ $$0P:(DE-Juel1)171786$$aSchulz, Jörg B.$$b9$$ufzj
000906834 7001_ $$0P:(DE-HGF)0$$aBach, Jan-Philipp$$b10
000906834 7001_ $$00000-0002-2387-526X$$aFalkenburger, Björn H.$$b11
000906834 7001_ $$0P:(DE-Juel1)166306$$aHoyer, Wolfgang$$b12$$eCorresponding author$$ufzj
000906834 773__ $$0PERI:(DE-600)2701262-1$$a10.3390/biom12030469$$gVol. 12, no. 3, p. 469 -$$n3$$p469 -$$tBiomolecules$$v12$$x2218-273X$$y2022
000906834 8564_ $$uhttps://juser.fz-juelich.de/record/906834/files/biomolecules-12-00469.pdf$$yOpenAccess
000906834 909CO $$ooai:juser.fz-juelich.de:906834$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000906834 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)165152$$aForschungszentrum Jülich$$b1$$kFZJ
000906834 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)171922$$aForschungszentrum Jülich$$b2$$kFZJ
000906834 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)176456$$aForschungszentrum Jülich$$b3$$kFZJ
000906834 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)132023$$aForschungszentrum Jülich$$b7$$kFZJ
000906834 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)132029$$aForschungszentrum Jülich$$b8$$kFZJ
000906834 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)171786$$aForschungszentrum Jülich$$b9$$kFZJ
000906834 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)166306$$aForschungszentrum Jülich$$b12$$kFZJ
000906834 9131_ $$0G:(DE-HGF)POF4-524$$1G:(DE-HGF)POF4-520$$2G:(DE-HGF)POF4-500$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-5244$$aDE-HGF$$bKey Technologies$$lNatural, Artificial and Cognitive Information Processing$$vMolecular and Cellular Information Processing$$x0
000906834 9141_ $$y2022
000906834 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000906834 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2021-05-04
000906834 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-05-04
000906834 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000906834 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2021-05-04
000906834 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-05-04
000906834 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2021-05-04
000906834 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bBIOMOLECULES : 2021$$d2022-11-22
000906834 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2022-11-22
000906834 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2022-11-22
000906834 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2022-01-07T14:30:28Z
000906834 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2022-01-07T14:30:28Z
000906834 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Blind peer review$$d2022-01-07T14:30:28Z
000906834 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2022-11-22
000906834 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2022-11-22
000906834 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2022-11-22
000906834 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2022-11-22
000906834 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2022-11-22
000906834 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bBIOMOLECULES : 2021$$d2022-11-22
000906834 9201_ $$0I:(DE-Juel1)IBI-7-20200312$$kIBI-7$$lStrukturbiochemie$$x0
000906834 9201_ $$0I:(DE-Juel1)INM-11-20170113$$kINM-11$$lJara-Institut Quantum Information$$x1
000906834 980__ $$ajournal
000906834 980__ $$aVDB
000906834 980__ $$aUNRESTRICTED
000906834 980__ $$aI:(DE-Juel1)IBI-7-20200312
000906834 980__ $$aI:(DE-Juel1)INM-11-20170113
000906834 9801_ $$aFullTexts