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@ARTICLE{Seide:906840,
      author       = {Seide, Selina and Arnold, Lilia and Wetzels, Solange and
                      Bregu, Mariela and Gätgens, Jochem and Pohl, Martina},
      title        = {{F}rom {E}nzyme to {P}reparative {C}ascade {R}eactions with
                      {I}mmobilized {E}nzymes: {T}uning
                      {F}e({II})/α-{K}etoglutarate-{D}ependent {L}ysine
                      {H}ydroxylases for {A}pplication in {B}iotransformations},
      journal      = {Catalysts},
      volume       = {12},
      number       = {4},
      issn         = {2073-4344},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2022-01734},
      pages        = {354 -},
      year         = {2022},
      abstract     = {Fe(II)/α-ketoglutarate-dependent dioxygenases (KDOs)
                      catalyze a broad range of selective C–H oxidation
                      reactions. However, the difficult production of KDOs in
                      recombinant E. coli strains and their instability in
                      purified form have so far limited their application in
                      preparative biotransformations. Here, we investigated the
                      immobilization of three KDOs (CaKDO, CpKDO, FjKDO) that
                      catalyze the stereoselective hydroxylation of the L-lysine
                      side chain using two one-step immobilization techniques
                      (HaloTag®, EziG™). The HaloTag®-based immobilisates
                      reached the best results with respect to residual activity
                      and stability. In preparative lab-scale experiments, we
                      achieved product titers of 16 g L−1 (3S)-hydroxy-L-lysine
                      (CaKDO) and (4R)-hydroxy-L-lysine (FjKDO), respectively,
                      starting from 100 mM L-lysine. Using a HaloTag®-immobilized
                      lysine decarboxylase from Selenomonas ruminantium (SrLDC),
                      the (3S)-hydroxy-L-lysine from the CaKDO-catalyzed reaction
                      was successfully converted to (2S)-hydroxy-cadaverine
                      without intermediate product purification, yielding a
                      product titer of 11.6 g L−1 in a 15 mL consecutive batch
                      reaction. We propose that covalent in situ immobilization is
                      an appropriate tool to access the preparative potential of
                      many other KDOs.},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000786882300001},
      doi          = {10.3390/catal12040354},
      url          = {https://juser.fz-juelich.de/record/906840},
}