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| 005 | 20230123110608.0 | ||
| 024 | 7 | _ | |a 10.3390/catal12040354 |2 doi |
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| 037 | _ | _ | |a FZJ-2022-01734 |
| 082 | _ | _ | |a 540 |
| 100 | 1 | _ | |a Seide, Selina |0 P:(DE-Juel1)173700 |b 0 |
| 245 | _ | _ | |a From Enzyme to Preparative Cascade Reactions with Immobilized Enzymes: Tuning Fe(II)/α-Ketoglutarate-Dependent Lysine Hydroxylases for Application in Biotransformations |
| 260 | _ | _ | |a Basel |c 2022 |b MDPI |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 520 | _ | _ | |a Fe(II)/α-ketoglutarate-dependent dioxygenases (KDOs) catalyze a broad range of selective C–H oxidation reactions. However, the difficult production of KDOs in recombinant E. coli strains and their instability in purified form have so far limited their application in preparative biotransformations. Here, we investigated the immobilization of three KDOs (CaKDO, CpKDO, FjKDO) that catalyze the stereoselective hydroxylation of the L-lysine side chain using two one-step immobilization techniques (HaloTag®, EziG™). The HaloTag®-based immobilisates reached the best results with respect to residual activity and stability. In preparative lab-scale experiments, we achieved product titers of 16 g L−1 (3S)-hydroxy-L-lysine (CaKDO) and (4R)-hydroxy-L-lysine (FjKDO), respectively, starting from 100 mM L-lysine. Using a HaloTag®-immobilized lysine decarboxylase from Selenomonas ruminantium (SrLDC), the (3S)-hydroxy-L-lysine from the CaKDO-catalyzed reaction was successfully converted to (2S)-hydroxy-cadaverine without intermediate product purification, yielding a product titer of 11.6 g L−1 in a 15 mL consecutive batch reaction. We propose that covalent in situ immobilization is an appropriate tool to access the preparative potential of many other KDOs. |
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| 588 | _ | _ | |a Dataset connected to CrossRef, Journals: juser.fz-juelich.de |
| 700 | 1 | _ | |a Arnold, Lilia |0 P:(DE-Juel1)144164 |b 1 |u fzj |
| 700 | 1 | _ | |a Wetzels, Solange |0 P:(DE-Juel1)180209 |b 2 |
| 700 | 1 | _ | |a Bregu, Mariela |0 P:(DE-Juel1)186705 |b 3 |
| 700 | 1 | _ | |a Gätgens, Jochem |0 P:(DE-Juel1)129023 |b 4 |
| 700 | 1 | _ | |a Pohl, Martina |0 P:(DE-Juel1)131522 |b 5 |e Corresponding author |
| 773 | _ | _ | |a 10.3390/catal12040354 |g Vol. 12, no. 4, p. 354 - |0 PERI:(DE-600)2662126-5 |n 4 |p 354 - |t Catalysts |v 12 |y 2022 |x 2073-4344 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/906840/files/catalysts-12-00354.pdf |y OpenAccess |
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