%0 Journal Article
%A Moe, Agnes
%A Kovalova, Terezia
%A Król, Sylwia
%A Yanofsky, David J.
%A Bott, Michael
%A Sjöstrand, Dan
%A Rubinstein, John L.
%A Högbom, Martin
%A Brzezinski, Peter
%T The respiratory supercomplex from C. glutamicum
%J Structure
%V 30
%N 3
%@ 0969-2126
%C Cambridge, Mass.
%I Cell Press
%M FZJ-2022-01888
%P 338 - 349.e3
%D 2022
%Z Biotechnologie 1
%X Corynebacterium glutamicum is a preferentially aerobic gram-positive bacterium belonging to the phylum Actinobacteria, which also includes the pathogen Mycobacterium tuberculosis. In these bacteria, respiratory complexes III and IV form a CIII2CIV2 supercomplex that catalyzes oxidation of menaquinol and reduction of dioxygen to water. We isolated the C. glutamicum supercomplex and used cryo-EM to determine its structure at 2.9 Å resolution. The structure shows a central CIII2 dimer flanked by a CIV on two sides. A menaquinone is bound in each of the QN and QP sites in each CIII and an additional menaquinone is positioned ∼14 Å from heme bL. A di-heme cyt. cc subunit electronically connects each CIII with an adjacent CIV, with the Rieske iron-sulfur protein positioned with the iron near heme bL. Multiple subunits interact to form a convoluted sub-structure at the cytoplasmic side of the supercomplex, which defines a path for proton transfer into CIV.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:34910901
%U <Go to ISI:>//WOS:000766494300005
%R 10.1016/j.str.2021.11.008
%U https://juser.fz-juelich.de/record/907200