TY  - JOUR
AU  - Moe, Agnes
AU  - Kovalova, Terezia
AU  - Król, Sylwia
AU  - Yanofsky, David J.
AU  - Bott, Michael
AU  - Sjöstrand, Dan
AU  - Rubinstein, John L.
AU  - Högbom, Martin
AU  - Brzezinski, Peter
TI  - The respiratory supercomplex from C. glutamicum
JO  - Structure
VL  - 30
IS  - 3
SN  - 0969-2126
CY  - Cambridge, Mass.
PB  - Cell Press
M1  - FZJ-2022-01888
SP  - 338 - 349.e3
PY  - 2022
N1  - Biotechnologie 1
AB  - Corynebacterium glutamicum is a preferentially aerobic gram-positive bacterium belonging to the phylum Actinobacteria, which also includes the pathogen Mycobacterium tuberculosis. In these bacteria, respiratory complexes III and IV form a CIII2CIV2 supercomplex that catalyzes oxidation of menaquinol and reduction of dioxygen to water. We isolated the C. glutamicum supercomplex and used cryo-EM to determine its structure at 2.9 Å resolution. The structure shows a central CIII2 dimer flanked by a CIV on two sides. A menaquinone is bound in each of the QN and QP sites in each CIII and an additional menaquinone is positioned ∼14 Å from heme bL. A di-heme cyt. cc subunit electronically connects each CIII with an adjacent CIV, with the Rieske iron-sulfur protein positioned with the iron near heme bL. Multiple subunits interact to form a convoluted sub-structure at the cytoplasmic side of the supercomplex, which defines a path for proton transfer into CIV.
LB  - PUB:(DE-HGF)16
C6  - pmid:34910901
UR  - <Go to ISI:>//WOS:000766494300005
DO  - DOI:10.1016/j.str.2021.11.008
UR  - https://juser.fz-juelich.de/record/907200
ER  -