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| Hauptseite > Publikationsdatenbank > The respiratory supercomplex from C. glutamicum > print |
| 001 | 907200 | ||
| 005 | 20230123110612.0 | ||
| 024 | 7 | _ | |a 10.1016/j.str.2021.11.008 |2 doi |
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| 245 | _ | _ | |a The respiratory supercomplex from C. glutamicum |
| 260 | _ | _ | |a Cambridge, Mass. |c 2022 |b Cell Press |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 500 | _ | _ | |a Biotechnologie 1 |
| 520 | _ | _ | |a Corynebacterium glutamicum is a preferentially aerobic gram-positive bacterium belonging to the phylum Actinobacteria, which also includes the pathogen Mycobacterium tuberculosis. In these bacteria, respiratory complexes III and IV form a CIII2CIV2 supercomplex that catalyzes oxidation of menaquinol and reduction of dioxygen to water. We isolated the C. glutamicum supercomplex and used cryo-EM to determine its structure at 2.9 Å resolution. The structure shows a central CIII2 dimer flanked by a CIV on two sides. A menaquinone is bound in each of the QN and QP sites in each CIII and an additional menaquinone is positioned ∼14 Å from heme bL. A di-heme cyt. cc subunit electronically connects each CIII with an adjacent CIV, with the Rieske iron-sulfur protein positioned with the iron near heme bL. Multiple subunits interact to form a convoluted sub-structure at the cytoplasmic side of the supercomplex, which defines a path for proton transfer into CIV. |
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| 700 | 1 | _ | |a Król, Sylwia |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Yanofsky, David J. |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Bott, Michael |0 P:(DE-Juel1)128943 |b 4 |
| 700 | 1 | _ | |a Sjöstrand, Dan |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Rubinstein, John L. |0 P:(DE-HGF)0 |b 6 |e Corresponding author |
| 700 | 1 | _ | |a Högbom, Martin |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Brzezinski, Peter |0 P:(DE-HGF)0 |b 8 |
| 773 | _ | _ | |a 10.1016/j.str.2021.11.008 |g Vol. 30, no. 3, p. 338 - 349.e3 |0 PERI:(DE-600)2031189-8 |n 3 |p 338 - 349.e3 |t Structure |v 30 |y 2022 |x 0969-2126 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/907200/files/1-s2.0-S0969212621004202-main.pdf |y OpenAccess |
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