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@ARTICLE{Menzel:907203,
author = {Menzel, Miriam and Reuter, Jan A. and Gräßel, David and
Costantini, Irene and Amunts, Katrin and Axer, Markus},
title = {{A}utomated computation of nerve fibre inclinations from
3{D} polarised light imaging measurements of brain tissue},
journal = {Scientific reports},
volume = {12},
number = {1},
issn = {2045-2322},
address = {[London]},
publisher = {Macmillan Publishers Limited, part of Springer Nature},
reportid = {FZJ-2022-01891},
pages = {4328},
year = {2022},
abstract = {The method 3D polarised light imaging (3D-PLI) measures the
birefringence of histological brain sections to determine
the spatial course of nerve fibres (myelinated axons). While
the in-plane fibre directions can be determined with high
accuracy, the computation of the out-of-plane fibre
inclinations is more challenging because they are derived
from the amplitude of the birefringence signals, which
depends e.g. on the amount of nerve fibres. One possibility
to improve the accuracy is to consider the average
transmitted light intensity (transmittance weighting). The
current procedure requires effortful manual adjustment of
parameters and anatomical knowledge. Here, we introduce an
automated, optimised computation of the fibre inclinations,
allowing for a much faster, reproducible determination of
fibre orientations in 3D-PLI. Depending on the degree of
myelination, the algorithm uses different models
(transmittance-weighted, unweighted, or a linear
combination), allowing to account for regionally specific
behaviour. As the algorithm is parallelised and GPU
optimised, it can be applied to large data sets. Moreover,
it only uses images from standard 3D-PLI measurements
without tilting, and can therefore be applied to existing
data sets from previous measurements. The functionality is
demonstrated on unstained coronal and sagittal histological
sections of vervet monkey and rat brains.},
cin = {INM-1},
ddc = {600},
cid = {I:(DE-Juel1)INM-1-20090406},
pnm = {5254 - Neuroscientific Data Analytics and AI (POF4-525) /
HBP SGA3 - Human Brain Project Specific Grant Agreement 3
(945539) / JL SMHB - Joint Lab Supercomputing and Modeling
for the Human Brain (JL SMHB-2021-2027)},
pid = {G:(DE-HGF)POF4-5254 / G:(EU-Grant)945539 / G:(DE-Juel1)JL
SMHB-2021-2027},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000769065000026},
doi = {10.1038/s41598-022-08140-0},
url = {https://juser.fz-juelich.de/record/907203},
}