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@ARTICLE{Dickerson:907441,
      author       = {Dickerson, Joshua L. and Lu, Penghan and Hristov, Dilyan
                      and Dunin-Borkowski, Rafal and Russo, Christopher J.},
      title        = {{I}maging biological macromolecules in thick specimens:
                      {T}he role of inelastic scattering in cryo{EM}},
      journal      = {Ultramicroscopy},
      volume       = {237},
      issn         = {0304-3991},
      address      = {Amsterdam},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2022-02039},
      pages        = {113510 -},
      year         = {2022},
      abstract     = {We investigate potential improvements in using electron
                      cryomicroscopy to image thick specimens with high-resolution
                      phase contrast imaging. In particular, using model
                      experiments, electron scattering theory, Monte Carlo and
                      multislice simulations, we determine the potential for
                      improving electron cryomicrographs of proteins within a cell
                      using chromatic aberration () correction. We show that
                      inelastically scattered electrons lose a quantifiable amount
                      of spatial coherence as they transit the specimen, yet can
                      be used to enhance the signal from thick biological
                      specimens (in the 1000 to 5000 Å range) provided they are
                      imaged close to focus with an achromatic lens. This loss of
                      information quantified here, which we call “specimen
                      induced decoherence”, is a fundamental limit on imaging
                      biological molecules in situ. We further show that with
                      foreseeable advances in transmission electron microscope
                      technology, it should be possible to directly locate and
                      uniquely identify sub-100 kDa proteins without the need for
                      labels, in a vitrified specimen taken from a cell.},
      cin          = {ER-C-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ER-C-1-20170209},
      pnm          = {5351 - Platform for Correlative, In Situ and Operando
                      Characterization (POF4-535)},
      pid          = {G:(DE-HGF)POF4-5351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:35367900},
      UT           = {WOS:000790826100002},
      doi          = {10.1016/j.ultramic.2022.113510},
      url          = {https://juser.fz-juelich.de/record/907441},
}