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@ARTICLE{Fricke:907814,
author = {Fricke, Philipp Moritz and Hartmann, Rudolf and Wirtz,
Astrid and Bott, Michael and Polen, Tino},
title = {{P}roduction of l-arabinonic acid from l-arabinose by the
acetic acid bacterium {G}luconobacter oxydans},
journal = {Bioresource technology reports},
volume = {17},
issn = {2589-014X},
address = {Amsterdam},
publisher = {Elsevier},
reportid = {FZJ-2022-02231},
pages = {100965 -},
year = {2022},
note = {Biotechnologie 1},
abstract = {l-Arabinonic acid is a five carbon sugar acid valuable for
various potential applications. Based on previous
observations that Gluconobacter oxydans 621H oxidizes
l-arabinose in the periplasm by the membrane-bound glucose
dehydrogenase GdhM yielding l-arabinonic acid, we tested its
ability for production. In fed-batch shake flasks without pH
control, 17.4 g/L l-arabinonic acid and 5.7 g/L
l-arabino-1,4-lactone were obtained. A large fraction of
l-arabinose remained unused since acidification of the
medium prevented further substrate oxidation. In pH
6-controlled fed-batch bioreactors, up to 120 g/L
l-arabinonic acid and 13 g/L l-arabino-1,4-lactone could be
obtained with strain 621H (144 h). Constitutive
overexpression of gdhM did not increase product titers and
resulted in reduced biomass formation, yet the gdhM
overexpression strain showed higher biomass-specific
production (169 g/L/gcdw) compared to 621H (131 g/L/gcdw).
The high combined product titer (133 g/L, 814 mM) makes G.
oxydans a very promising host for high-level production of
l-arabinonic acid.},
cin = {IBG-1},
ddc = {570},
cid = {I:(DE-Juel1)IBG-1-20101118},
pnm = {2172 - Utilization of renewable carbon and energy sources
and engineering of ecosystem functions (POF4-217)},
pid = {G:(DE-HGF)POF4-2172},
typ = {PUB:(DE-HGF)16},
UT = {WOS:001206651900021},
doi = {10.1016/j.biteb.2022.100965},
url = {https://juser.fz-juelich.de/record/907814},
}