% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{lc:907898,
      author       = {Ölçücü, Gizem and Baumer, Benedikt and Küsters, Kira
                      and Möllenhoff, Kathrin and Oldiges, Marco and Pietruszka,
                      Jörg and Jaeger, Karl-Erich and Krauss, Ulrich},
      title        = {{C}atalytically {A}ctive {I}nclusion
                      {B}odies─{B}enchmarking and {A}pplication in {F}low
                      {C}hemistry},
      journal      = {ACS synthetic biology},
      volume       = {11},
      number       = {5},
      issn         = {2161-5063},
      address      = {Washington, DC},
      publisher    = {ACS},
      reportid     = {FZJ-2022-02276},
      pages        = {1881 - 1896},
      year         = {2022},
      abstract     = {In industries, enzymes are often immobilized to obtain
                      stable preparations that can be utilized in batch and flow
                      processes. In contrast to traditional immobilization methods
                      that rely on carrier binding, various immobilization
                      strategies have been recently presented that enable the
                      simultaneous production and in vivo immobilization of
                      enzymes. Catalytically active inclusion bodies (CatIBs) are
                      a promising example for such in vivo enzyme immobilizates.
                      CatIB formation is commonly induced by fusion of
                      aggregation-inducing tags, and numerous tags, ranging from
                      small synthetic peptides to protein domains or whole
                      proteins, have been successfully used. However, since these
                      systems have been characterized by different groups
                      employing different methods, a direct comparison remains
                      difficult, which prompted us to benchmark different
                      CatIB-formation-inducing tags and fusion strategies. Our
                      study highlights that important CatIB properties like yield,
                      activity, and stability are strongly influenced by tag
                      selection and fusion strategy. Optimization enabled us to
                      obtain alcohol dehydrogenase CatIBs with superior activity
                      and stability, which were subsequently applied for the first
                      time in a flow synthesis approach. Our study highlights the
                      potential of CatIB-based immobilizates, while at the same
                      time demonstrating the robust use of CatIBs in flow
                      chemistry.},
      cin          = {IBG-1 / IMET / IBOC},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118 / I:(DE-Juel1)IMET-20090612 /
                      I:(DE-Juel1)IBOC-20090406},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:35500299},
      UT           = {WOS:000805370400018},
      doi          = {10.1021/acssynbio.2c00035},
      url          = {https://juser.fz-juelich.de/record/907898},
}