% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Kempmann:908173,
      author       = {Kempmann, Annika and Gensch, Thomas and Offenhäusser,
                      Andreas and Tihaa, Irina and Maybeck, Vanessa and Balfanz,
                      Sabine and Baumann, Arnd},
      title        = {{T}he {F}unctional {C}haracterization of {GC}a{MP}3.0
                      {V}ariants {S}pecifically {T}argeted to {S}ubcellular
                      {D}omains},
      journal      = {International journal of molecular sciences},
      volume       = {23},
      number       = {12},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {FZJ-2022-02431},
      pages        = {6593 -},
      year         = {2022},
      abstract     = {Calcium (Ca2+) ions play a pivotal role in physiology and
                      cellular signaling. The intracellular Ca2+ concentration
                      ([Ca2+]i) is about three orders of magnitude lower than the
                      extracellular concentration, resulting in a steep
                      transmembrane concentration gradient. Thus, the spatial and
                      the temporal dynamics of [Ca2+]i are ideally suited to
                      modulate Ca2+-mediated cellular responses to external
                      signals. A variety of highly sophisticated methods have been
                      developed to gain insight into cellular Ca2+ dynamics. In
                      addition to electrophysiological measurements and the
                      application of synthetic dyes that change their fluorescent
                      properties upon interaction with Ca2+, the introduction and
                      the ongoing development of genetically encoded Ca2+
                      indicators (GECI) opened a new era to study Ca2+-driven
                      processes in living cells and organisms. Here, we have
                      focused on one well-established GECI, i.e., GCaMP3.0. We
                      have systematically modified the protein with sequence
                      motifs, allowing localization of the sensor in the nucleus,
                      in the mitochondrial matrix, at the mitochondrial outer
                      membrane, and at the plasma membrane. The individual
                      variants and a cytosolic version of GCaMP3.0 were
                      overexpressed and purified from E. coli cells to study their
                      biophysical properties in solution. All versions were
                      examined to monitor Ca2+ signaling in stably transfected
                      cell lines and in primary cortical neurons transduced with
                      recombinant Adeno-associated viruses (rAAV). In this
                      comparative study, we provide evidence for a robust approach
                      to reliably trace Ca2+ signals at the (sub)-cellular level
                      with pronounced temporal resolution},
      cin          = {IBI-1 / IBI-3},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-1-20200312 / I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {35743038},
      UT           = {WOS:000816484300001},
      doi          = {10.3390/ijms23126593},
      url          = {https://juser.fz-juelich.de/record/908173},
}