%0 Journal Article
%A Kuang, Weiqi
%A Sanow, Stefan
%A Kelm, Jana M
%A Müller Linow, Mark
%A Andeer, Peter
%A Kohlheyer, Dietrich
%A Northen, Trent
%A Vogel, John P
%A Watt, Michelle
%A Arsova, Borjana
%T N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon
%J The journal of experimental botany
%V 73
%N 15
%@ 0022-0957
%C Oxford
%I Oxford Univ. Press
%M FZJ-2022-02502
%P 5306–5321
%D 2022
%X Nitrogen (N) fixation in cereals by root-associated bacteria is a promising solution for reducing use of chemical N fertilizers in agriculture. However, plant and bacterial responses are unpredictable across environments. We hypothesized that cereal responses to N-fixing bacteria are dynamic, depending on N supply and time. To quantify the dynamics, a gnotobiotic, fabricated ecosystem (EcoFAB) was adapted to analyse N mass balance, to image shoot and root growth, and to measure gene expression of Brachypodium distachyon inoculated with the N-fixing bacterium Herbaspirillum seropedicae. Phenotyping throughput of EcoFAB-N was 25–30 plants h−1 with open software and imaging systems. Herbaspirillum seropedicae inoculation of B. distachyon shifted root and shoot growth, nitrate versus ammonium uptake, and gene expression with time; directions and magnitude depended on N availability. Primary roots were longer and root hairs shorter regardless of N, with stronger changes at low N. At higher N, H. seropedicae provided 11% of the total plant N that came from sources other than the seed or the nutrient solution. The time-resolved phenotypic and molecular data point to distinct modes of action: at 5 mM NH4NO3 the benefit appears through N fixation, while at 0.5 mM NH4NO3 the mechanism appears to be plant physiological, with H. seropedicae promoting uptake of N from the root medium.Future work could fine-tune plant and root-associated microorganisms to growth and nutrient dynamics.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 35512445
%U <Go to ISI:>//WOS:000813473600001
%R 10.1093/jxb/erac184
%U https://juser.fz-juelich.de/record/908268