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@ARTICLE{Kuang:908268,
      author       = {Kuang, Weiqi and Sanow, Stefan and Kelm, Jana M and Müller
                      Linow, Mark and Andeer, Peter and Kohlheyer, Dietrich and
                      Northen, Trent and Vogel, John P and Watt, Michelle and
                      Arsova, Borjana},
      title        = {{N}-dependent dynamics of root growth and nitrate and
                      ammonium uptake are altered by the bacterium
                      {H}erbaspirillum seropedicae in the cereal model
                      {B}rachypodium distachyon},
      journal      = {The journal of experimental botany},
      volume       = {73},
      number       = {15},
      issn         = {0022-0957},
      address      = {Oxford},
      publisher    = {Oxford Univ. Press},
      reportid     = {FZJ-2022-02502},
      pages        = {5306–5321},
      year         = {2022},
      abstract     = {Nitrogen (N) fixation in cereals by root-associated
                      bacteria is a promising solution for reducing use of
                      chemical N fertilizers in agriculture. However, plant and
                      bacterial responses are unpredictable across environments.
                      We hypothesized that cereal responses to N-fixing bacteria
                      are dynamic, depending on N supply and time. To quantify the
                      dynamics, a gnotobiotic, fabricated ecosystem (EcoFAB) was
                      adapted to analyse N mass balance, to image shoot and root
                      growth, and to measure gene expression of Brachypodium
                      distachyon inoculated with the N-fixing bacterium
                      Herbaspirillum seropedicae. Phenotyping throughput of
                      EcoFAB-N was 25–30 plants h−1 with open software and
                      imaging systems. Herbaspirillum seropedicae inoculation of
                      B. distachyon shifted root and shoot growth, nitrate versus
                      ammonium uptake, and gene expression with time; directions
                      and magnitude depended on N availability. Primary roots were
                      longer and root hairs shorter regardless of N, with stronger
                      changes at low N. At higher N, H. seropedicae provided
                      $11\%$ of the total plant N that came from sources other
                      than the seed or the nutrient solution. The time-resolved
                      phenotypic and molecular data point to distinct modes of
                      action: at 5 mM NH4NO3 the benefit appears through N
                      fixation, while at 0.5 mM NH4NO3 the mechanism appears to be
                      plant physiological, with H. seropedicae promoting uptake of
                      N from the root medium.Future work could fine-tune plant and
                      root-associated microorganisms to growth and nutrient
                      dynamics.},
      cin          = {IBG-2 / IBG-1},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118 / I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {35512445},
      UT           = {WOS:000813473600001},
      doi          = {10.1093/jxb/erac184},
      url          = {https://juser.fz-juelich.de/record/908268},
}