Gast ::
| Hauptseite > Publikationsdatenbank > Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation > print |
| Hauptseite > Publikationsdatenbank > Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation > print |
| 001 | 908892 | ||
| 005 | 20230301072907.0 | ||
| 024 | 7 | _ | |a 10.1007/s00253-022-12017-7 |2 doi |
| 024 | 7 | _ | |a 0171-1741 |2 ISSN |
| 024 | 7 | _ | |a 0175-7598 |2 ISSN |
| 024 | 7 | _ | |a 0340-2118 |2 ISSN |
| 024 | 7 | _ | |a 1432-0614 |2 ISSN |
| 024 | 7 | _ | |a 2128/31591 |2 Handle |
| 024 | 7 | _ | |a 35759036 |2 pmid |
| 024 | 7 | _ | |a WOS:000817028300003 |2 WOS |
| 037 | _ | _ | |a FZJ-2022-02899 |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Müller, Carolin |0 P:(DE-Juel1)177776 |b 0 |u fzj |
| 245 | _ | _ | |a Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation |
| 260 | _ | _ | |a New York |c 2022 |b Springer |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1659086000_17463 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Secretion of bacterial proteins into the culture medium simplifies downstream processing by avoiding cell disruption for target protein purification. However, a suitable signal peptide for efficient secretion needs to be identified, and currently, there are no tools available to predict optimal combinations of signal peptides and target proteins. The selection of such a combination is influenced by several factors, including protein biosynthesis efficiency and cultivation conditions, which both can have a significant impact on secretion performance. As a result, a large number of combinations must be tested. Therefore, we have developed automated workflows allowing for targeted strain construction and secretion screening using two platforms. Key advantages of this experimental setup include lowered hands-on time and increased throughput. In this study, the automated workflows were established for the heterologous production of Fusarium solani f. sp. pisi cutinase in Corynebacterium glutamicum. The target protein was monitored in culture supernatants via enzymatic activity and split GFP assay. Varying spacer lengths between the Shine-Dalgarno sequence and the start codon of Bacillus subtilis signal peptides were tested. Consistent with previous work on the secretory cutinase production in B. subtilis, a ribosome binding site with extended spacer length to up to 12 nt, which likely slows down translation initiation, does not necessarily lead to poorer cutinase secretion by C. glutamicum. The best performing signal peptides for cutinase secretion with a standard spacer length were identified in a signal peptide screening. Additional insights into the secretion process were gained by monitoring secretion stress using the C. glutamicum K9 biosensor strain. |
| 536 | _ | _ | |a 2172 - Utilization of renewable carbon and energy sources and engineering of ecosystem functions (POF4-217) |0 G:(DE-HGF)POF4-2172 |c POF4-217 |f POF IV |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef, Journals: juser.fz-juelich.de |
| 700 | 1 | _ | |a Bakkes, Patrick J. |0 P:(DE-Juel1)176296 |b 1 |u fzj |
| 700 | 1 | _ | |a Lenz, Patrick |0 P:(DE-Juel1)171683 |b 2 |u fzj |
| 700 | 1 | _ | |a Waffenschmidt, Vera |0 P:(DE-Juel1)186097 |b 3 |
| 700 | 1 | _ | |a Helleckes, Laura M. |0 P:(DE-Juel1)178687 |b 4 |u fzj |
| 700 | 1 | _ | |a Jaeger, Karl-Erich |0 P:(DE-Juel1)131457 |b 5 |u fzj |
| 700 | 1 | _ | |a Wiechert, Wolfgang |0 P:(DE-Juel1)129076 |b 6 |u fzj |
| 700 | 1 | _ | |a Knapp, Andreas |0 P:(DE-Juel1)131469 |b 7 |
| 700 | 1 | _ | |a Freudl, Roland |0 P:(DE-Juel1)128960 |b 8 |u fzj |
| 700 | 1 | _ | |a Oldiges, Marco |0 P:(DE-Juel1)129053 |b 9 |e Corresponding author |
| 773 | _ | _ | |a 10.1007/s00253-022-12017-7 |g Vol. 106, no. 12, p. 4481 - 4497 |0 PERI:(DE-600)1464336-4 |n 12 |p 4481 - 4497 |t Applied microbiology and biotechnology |v 106 |y 2022 |x 0171-1741 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/908892/files/M%C3%BCller2022_Article_AcceleratedStrainConstructionA.pdf |y OpenAccess |
| 909 | C | O | |o oai:juser.fz-juelich.de:908892 |p openaire |p open_access |p OpenAPC_DEAL |p driver |p VDB |p openCost |p dnbdelivery |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 0 |6 P:(DE-Juel1)177776 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 1 |6 P:(DE-Juel1)176296 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 2 |6 P:(DE-Juel1)171683 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 4 |6 P:(DE-Juel1)178687 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 5 |6 P:(DE-Juel1)131457 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 6 |6 P:(DE-Juel1)129076 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 8 |6 P:(DE-Juel1)128960 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 9 |6 P:(DE-Juel1)129053 |
| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Erde und Umwelt |l Erde im Wandel – Unsere Zukunft nachhaltig gestalten |1 G:(DE-HGF)POF4-210 |0 G:(DE-HGF)POF4-217 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-200 |4 G:(DE-HGF)POF |v Für eine nachhaltige Bio-Ökonomie – von Ressourcen zu Produkten |9 G:(DE-HGF)POF4-2172 |x 0 |
| 914 | 1 | _ | |y 2022 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0160 |2 StatID |b Essential Science Indicators |d 2021-01-30 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1190 |2 StatID |b Biological Abstracts |d 2021-01-30 |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 915 | _ | _ | |a DEAL Springer |0 StatID:(DE-HGF)3002 |2 StatID |d 2021-01-30 |w ger |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0113 |2 StatID |b Science Citation Index Expanded |d 2021-01-30 |
| 915 | _ | _ | |a Creative Commons Attribution CC BY 4.0 |0 LIC:(DE-HGF)CCBY4 |2 HGFVOC |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b APPL MICROBIOL BIOT : 2021 |d 2022-11-12 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2022-11-12 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2022-11-12 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0600 |2 StatID |b Ebsco Academic Search |d 2022-11-12 |
| 915 | _ | _ | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b ASC |d 2022-11-12 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2022-11-12 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2022-11-12 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |d 2022-11-12 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |d 2022-11-12 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1060 |2 StatID |b Current Contents - Agriculture, Biology and Environmental Sciences |d 2022-11-12 |
| 915 | _ | _ | |a IF >= 5 |0 StatID:(DE-HGF)9905 |2 StatID |b APPL MICROBIOL BIOT : 2021 |d 2022-11-12 |
| 915 | p | c | |a APC keys set |2 APC |0 PC:(DE-HGF)0000 |
| 915 | p | c | |a Local Funding |2 APC |0 PC:(DE-HGF)0001 |
| 915 | p | c | |a DFG OA Publikationskosten |2 APC |0 PC:(DE-HGF)0002 |
| 915 | p | c | |a DEAL: Springer Nature 2020 |2 APC |0 PC:(DE-HGF)0113 |
| 920 | 1 | _ | |0 I:(DE-Juel1)IBG-1-20101118 |k IBG-1 |l Biotechnologie |x 0 |
| 920 | 1 | _ | |0 I:(DE-Juel1)IMET-20090612 |k IMET |l Institut für Molekulare Enzymtechnologie (HHUD) |x 1 |
| 980 | 1 | _ | |a FullTexts |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)IBG-1-20101118 |
| 980 | _ | _ | |a I:(DE-Juel1)IMET-20090612 |
| 980 | _ | _ | |a APC |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|