TY  - JOUR
AU  - Laschke, Laura
AU  - Schütz, Vadim
AU  - Schackow, Oliver
AU  - Sicker, Dieter
AU  - Hennig, Lothar
AU  - Hofmann, Diana
AU  - Dörmann, Peter
AU  - Schulz, Margot
TI  - Survival of Plants During Short-Term BOA-OH Exposure: ROS Related Gene Expression and Detoxification Reactions Are Accompanied With Fast Membrane Lipid Repair in Root Tips
JO  - Journal of chemical ecology
VL  - 48
IS  - 2
SN  - 0098-0331
CY  - New York, NY [u.a.]
PB  - Springer Science + Business Media B.V.
M1  - FZJ-2022-02977
SP  - 219 - 239
PY  - 2022
AB  - For the characterization of BOA-OH insensitive plants, we studied the time-dependent effects of the benzoxazolinone-4/5/6/7-OH isomers on maize roots. Exposure of Zea mays seedlings to 0.5 mM BOA-OH elicits root zone-specific reactions bythe formation of dark rings and spots in the zone of lateral roots, high catalase activity on root hairs, and no visible defensereaction at the root tip. We studied BOA-6-OH- short-term effects on membrane lipids and fatty acids in maize root tips incomparison to the benzoxazinone-free species Abutilon theophrasti Medik. Decreased contents of phosphatidylinositol inA. theophrasti and phosphatidylcholine in maize were found after 10–30 min. In the youngest tissue, α-linoleic acid (18:2),decreased considerably in both species and recovered within one hr. Disturbances in membrane phospholipid contents werebalanced in both species within 30–60 min. Triacylglycerols (TAGs) were also affected, but levels of maize diacylglycer-ols (DAGs) were almost unchanged, suggesting a release of fatty acids for membrane lipid regeneration from TAGs whileresulting DAGs are buildings blocks for phospholipid reconstitution, concomitant with BOA-6-OH glucosylation. Expres-sion of superoxide dismutase (SOD2) and of ER-bound oleoyl desaturase (FAD2-2) genes were contemporaneously upregulated in contrast to the catalase CAT1, while CAT3 was arguably involved at a later stage of the detoxification process.Immuno-responses were not elicited in short-terms, since the expression of NPR1, POX12 were barely affected, PR4 after6 h with BOA-4/7-OH and PR1 after 24 h with BOA-5/6-OH. The rapid membrane recovery, reactive oxygen species, andallelochemical detoxification may be characteristic for BOA-OH insensitive plants.
LB  - PUB:(DE-HGF)16
C6  - 34988771
UR  - <Go to ISI:>//WOS:000739233600001
DO  - DOI:10.1007/s10886-021-01337-z
UR  - https://juser.fz-juelich.de/record/909039
ER  -