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@ARTICLE{Schito:909084,
      author       = {Schito, Simone and Zuchowski, Rico and Bergen, Daniel and
                      Strohmeier, Daniel and Wollenhaupt, Bastian and Menke,
                      Philipp and Seiffarth, Johannes and Nöh, Katharina and
                      Kohlheyer, Dietrich and Bott, Michael and Wiechert, Wolfgang
                      and Baumgart, Meike and Noack, Stephan},
      title        = {{C}ommunities of {N}iche-optimized {S}trains ({C}o{N}o{S})
                      – {D}esign and creation of stable, genome-reduced
                      co-cultures},
      journal      = {Metabolic engineering},
      volume       = {73},
      issn         = {1096-7176},
      address      = {Orlando, Fla.},
      publisher    = {Academic Press},
      reportid     = {FZJ-2022-03001},
      pages        = {91 - 103},
      year         = {2022},
      abstract     = {Current bioprocesses for production of value-added
                      compounds are mainly based on pure cultures that are
                      composed of rationally engineered strains of model organisms
                      with versatile metabolic capacities. However, in the
                      comparably well-defined environment of a bioreactor,
                      metabolic flexibility provided by various highly abundant
                      biosynthetic enzymes is much less required and results in
                      suboptimal use of carbon and energy sources for compound
                      production. In nature, non-model organisms have frequently
                      evolved in communities where genome-reduced, auxotrophic
                      strains cross-feed each other, suggesting that there must be
                      a significant advantage compared to growth without
                      cooperation. To prove this, we started to create and study
                      synthetic communities of niche-optimized strains (CoNoS)
                      that consists of two strains of the same species
                      Corynebacterium glutamicum that are mutually dependent on
                      one amino acid. We used both the wild-type and the
                      genome-reduced C1* chassis for introducing selected amino
                      acid auxotrophies, each based on complete deletion of all
                      required biosynthetic genes. The best candidate strains were
                      used to establish several stably growing CoNoS that were
                      further characterized and optimized by metabolic modelling,
                      microfluidic experiments and rational metabolic engineering
                      to improve amino acid production and exchange. Finally, the
                      engineered CoNoS consisting of an l-leucine and l-arginine
                      auxotroph showed a specific growth rate equivalent to $83\%$
                      of the wild type in monoculture, making it the fastest
                      co-culture of two auxotrophic C. glutamicum strains to date.
                      Overall, our results are a first promising step towards
                      establishing improved biobased production of value-added
                      compounds using the CoNoS approach.},
      cin          = {IBG-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217) / DFG project 428038451 - SiMBal
                      2.0: Quantifizierung der Co-Kultur-Leistung und der
                      intrazellulären Interaktionen in Abhängigkeit der
                      Umgebung},
      pid          = {G:(DE-HGF)POF4-2171 / G:(GEPRIS)428038451},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {35750243},
      UT           = {WOS:001279119000001},
      doi          = {10.1016/j.ymben.2022.06.004},
      url          = {https://juser.fz-juelich.de/record/909084},
}