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@ARTICLE{Devan:909232,
author = {Devan, Senthil-Kumar and Schott-Verdugo, Stephan and
Müntjes, Kira and Bismar, Lilli and Reiners, Jens and
Hachani, Eymen and Schmitt, Lutz and Höppner, Astrid and
Smits, Sander HJ and Gohlke, Holger and Feldbrügge,
Michael},
title = {{A} {M}ademoise{LLE} domain binding platform links the key
{RNA} transporter to endosomes},
journal = {PLoS Genetics},
volume = {18},
number = {6},
issn = {1553-7390},
address = {San Francisco, Calif.},
publisher = {Public Library of Science},
reportid = {FZJ-2022-03076},
pages = {e1010269 -},
year = {2022},
abstract = {Spatiotemporal expression can be achieved by transport and
translation of mRNAs at defined subcellular sites. An
emerging mechanism mediating mRNA trafficking is
microtubule-dependent co-transport on shuttling endosomes.
Although progress has been made in identifying various
components of the endosomal mRNA transport machinery, a
mechanistic understanding of how these RNA-binding proteins
are connected to endosomes is still lacking. Here, we
demonstrate that a flexible MademoiseLLE (MLLE) domain
platform within RNA-binding protein Rrm4 of Ustilago maydis
is crucial for endosomal attachment. Our structure/function
analysis uncovered three MLLE domains at the C-terminus of
Rrm4 with a functionally defined hierarchy. MLLE3 recognises
two PAM2-like sequences of the adaptor protein Upa1 and is
essential for endosomal shuttling of Rrm4. MLLE1 and MLLE2
are most likely accessory domains exhibiting a variable
binding mode for interaction with currently unknown
partners. Thus, endosomal attachment of the mRNA transporter
is orchestrated by a sophisticated MLLE domain binding
platform.},
cin = {JSC / NIC / IBI-7 / IBG-4},
ddc = {610},
cid = {I:(DE-Juel1)JSC-20090406 / I:(DE-Juel1)NIC-20090406 /
I:(DE-Juel1)IBI-7-20200312 / I:(DE-Juel1)IBG-4-20200403},
pnm = {5111 - Domain-Specific Simulation $\&$ Data Life Cycle Labs
(SDLs) and Research Groups (POF4-511) / 2171 - Biological
and environmental resources for sustainable use (POF4-217) /
Forschergruppe Gohlke $(hkf7_20200501)$ / 5241 - Molecular
Information Processing in Cellular Systems (POF4-524)},
pid = {G:(DE-HGF)POF4-5111 / G:(DE-HGF)POF4-2171 /
$G:(DE-Juel1)hkf7_20200501$ / G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {35727840},
UT = {WOS:000828680400007},
doi = {10.1371/journal.pgen.1010269},
url = {https://juser.fz-juelich.de/record/909232},
}