%0 Journal Article
%A Lazić, Ivan
%A Wirix, Maarten
%A Leidl, Max Leo
%A de Haas, Felix
%A Mann, Daniel
%A Beckers, Maximilian
%A Pechnikova, Evgeniya V.
%A Müller-Caspary, Knut
%A Egoavil, Ricardo
%A Bosch, Eric G. T.
%A Sachse, Carsten
%T Single-particle cryo-EM structures from iDPC–STEM at near-atomic resolution
%J Nature methods
%V 1
%@ 1548-7091
%C London [u.a.]
%I Nature Publishing Group
%M FZJ-2022-03323
%P 38
%D 2022
%X In electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM) using large underfocuses and subsequently computationally combined into a high-resolution three-dimensional structure. Here, we apply scanning transmission electron microscopy (STEM) using the integrated differential phase contrast mode also known as iDPC–STEM to two cryo-EM test specimens, keyhole limpet hemocyanin (KLH) and tobacco mosaic virus (TMV). The micrographs show complete contrast transfer to high resolution and enable the cryo-EM structure determination for KLH at 6.5 Å resolution, as well as for TMV at 3.5 Å resolution using single-particle reconstruction methods, which share identical features with maps obtained by CTEM of a previously acquired same-sized TMV data set. These data show that STEM imaging in general, and in particular the iDPC–STEM approach, can be applied to vitrified single-particle specimens to determine near-atomic resolution cryo-EM structures of biological macromolecules.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 36064775
%U <Go to ISI:>//WOS:000850014200003
%R 10.1038/s41592-022-01586-0
%U https://juser.fz-juelich.de/record/909655