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000909655 1001_ $$00000-0001-5238-952X$$aLazić, Ivan$$b0
000909655 245__ $$aSingle-particle cryo-EM structures from iDPC–STEM at near-atomic resolution
000909655 260__ $$aLondon [u.a.]$$bNature Publishing Group$$c2022
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000909655 520__ $$aIn electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM) using large underfocuses and subsequently computationally combined into a high-resolution three-dimensional structure. Here, we apply scanning transmission electron microscopy (STEM) using the integrated differential phase contrast mode also known as iDPC–STEM to two cryo-EM test specimens, keyhole limpet hemocyanin (KLH) and tobacco mosaic virus (TMV). The micrographs show complete contrast transfer to high resolution and enable the cryo-EM structure determination for KLH at 6.5 Å resolution, as well as for TMV at 3.5 Å resolution using single-particle reconstruction methods, which share identical features with maps obtained by CTEM of a previously acquired same-sized TMV data set. These data show that STEM imaging in general, and in particular the iDPC–STEM approach, can be applied to vitrified single-particle specimens to determine near-atomic resolution cryo-EM structures of biological macromolecules.
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000909655 7001_ $$0P:(DE-HGF)0$$aWirix, Maarten$$b1
000909655 7001_ $$0P:(DE-Juel1)186015$$aLeidl, Max Leo$$b2$$ufzj
000909655 7001_ $$0P:(DE-HGF)0$$ade Haas, Felix$$b3
000909655 7001_ $$0P:(DE-Juel1)179550$$aMann, Daniel$$b4$$eCorresponding author
000909655 7001_ $$0P:(DE-Juel1)177870$$aBeckers, Maximilian$$b5
000909655 7001_ $$0P:(DE-HGF)0$$aPechnikova, Evgeniya V.$$b6
000909655 7001_ $$0P:(DE-Juel1)165314$$aMüller-Caspary, Knut$$b7$$ufzj
000909655 7001_ $$0P:(DE-HGF)0$$aEgoavil, Ricardo$$b8
000909655 7001_ $$0P:(DE-HGF)0$$aBosch, Eric G. T.$$b9
000909655 7001_ $$0P:(DE-Juel1)173949$$aSachse, Carsten$$b10$$eCorresponding author
000909655 773__ $$0PERI:(DE-600)2163081-1$$a10.1038/s41592-022-01586-0$$p38$$tNature methods$$v1$$x1548-7091$$y2022
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