TY  - JOUR
AU  - Lazić, Ivan
AU  - Wirix, Maarten
AU  - Leidl, Max Leo
AU  - de Haas, Felix
AU  - Mann, Daniel
AU  - Beckers, Maximilian
AU  - Pechnikova, Evgeniya V.
AU  - Müller-Caspary, Knut
AU  - Egoavil, Ricardo
AU  - Bosch, Eric G. T.
AU  - Sachse, Carsten
TI  - Single-particle cryo-EM structures from iDPC–STEM at near-atomic resolution
JO  - Nature methods
VL  - 1
SN  - 1548-7091
CY  - London [u.a.]
PB  - Nature Publishing Group
M1  - FZJ-2022-03323
SP  - 38
PY  - 2022
AB  - In electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM) using large underfocuses and subsequently computationally combined into a high-resolution three-dimensional structure. Here, we apply scanning transmission electron microscopy (STEM) using the integrated differential phase contrast mode also known as iDPC–STEM to two cryo-EM test specimens, keyhole limpet hemocyanin (KLH) and tobacco mosaic virus (TMV). The micrographs show complete contrast transfer to high resolution and enable the cryo-EM structure determination for KLH at 6.5 Å resolution, as well as for TMV at 3.5 Å resolution using single-particle reconstruction methods, which share identical features with maps obtained by CTEM of a previously acquired same-sized TMV data set. These data show that STEM imaging in general, and in particular the iDPC–STEM approach, can be applied to vitrified single-particle specimens to determine near-atomic resolution cryo-EM structures of biological macromolecules.
LB  - PUB:(DE-HGF)16
C6  - 36064775
UR  - <Go to ISI:>//WOS:000850014200003
DO  - DOI:10.1038/s41592-022-01586-0
UR  - https://juser.fz-juelich.de/record/909655
ER  -