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@ARTICLE{Lazi:909655,
      author       = {Lazić, Ivan and Wirix, Maarten and Leidl, Max Leo and de
                      Haas, Felix and Mann, Daniel and Beckers, Maximilian and
                      Pechnikova, Evgeniya V. and Müller-Caspary, Knut and
                      Egoavil, Ricardo and Bosch, Eric G. T. and Sachse, Carsten},
      title        = {{S}ingle-particle cryo-{EM} structures from i{DPC}–{STEM}
                      at near-atomic resolution},
      journal      = {Nature methods},
      volume       = {1},
      issn         = {1548-7091},
      address      = {London [u.a.]},
      publisher    = {Nature Publishing Group},
      reportid     = {FZJ-2022-03323},
      pages        = {38},
      year         = {2022},
      abstract     = {In electron cryomicroscopy (cryo-EM), molecular images of
                      vitrified biological samples are obtained by conventional
                      transmission microscopy (CTEM) using large underfocuses and
                      subsequently computationally combined into a high-resolution
                      three-dimensional structure. Here, we apply scanning
                      transmission electron microscopy (STEM) using the integrated
                      differential phase contrast mode also known as iDPC–STEM
                      to two cryo-EM test specimens, keyhole limpet hemocyanin
                      (KLH) and tobacco mosaic virus (TMV). The micrographs show
                      complete contrast transfer to high resolution and enable the
                      cryo-EM structure determination for KLH at 6.5 Å
                      resolution, as well as for TMV at 3.5 Å resolution using
                      single-particle reconstruction methods, which share
                      identical features with maps obtained by CTEM of a
                      previously acquired same-sized TMV data set. These data show
                      that STEM imaging in general, and in particular the
                      iDPC–STEM approach, can be applied to vitrified
                      single-particle specimens to determine near-atomic
                      resolution cryo-EM structures of biological macromolecules.},
      cin          = {ER-C-3},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ER-C-3-20170113},
      pnm          = {5352 - Understanding the Functionality of Soft Matter and
                      Biomolecular Systems (POF4-535) / 5241 - Molecular
                      Information Processing in Cellular Systems (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5352 / G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {36064775},
      UT           = {WOS:000850014200003},
      doi          = {10.1038/s41592-022-01586-0},
      url          = {https://juser.fz-juelich.de/record/909655},
}