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| 001 | 909655 | ||
| 005 | 20240627202007.0 | ||
| 024 | 7 | _ | |a 10.1038/s41592-022-01586-0 |2 doi |
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| 100 | 1 | _ | |a Lazić, Ivan |0 0000-0001-5238-952X |b 0 |
| 245 | _ | _ | |a Single-particle cryo-EM structures from iDPC–STEM at near-atomic resolution |
| 260 | _ | _ | |a London [u.a.] |c 2022 |b Nature Publishing Group |
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| 520 | _ | _ | |a In electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM) using large underfocuses and subsequently computationally combined into a high-resolution three-dimensional structure. Here, we apply scanning transmission electron microscopy (STEM) using the integrated differential phase contrast mode also known as iDPC–STEM to two cryo-EM test specimens, keyhole limpet hemocyanin (KLH) and tobacco mosaic virus (TMV). The micrographs show complete contrast transfer to high resolution and enable the cryo-EM structure determination for KLH at 6.5 Å resolution, as well as for TMV at 3.5 Å resolution using single-particle reconstruction methods, which share identical features with maps obtained by CTEM of a previously acquired same-sized TMV data set. These data show that STEM imaging in general, and in particular the iDPC–STEM approach, can be applied to vitrified single-particle specimens to determine near-atomic resolution cryo-EM structures of biological macromolecules. |
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| 700 | 1 | _ | |a Mann, Daniel |0 P:(DE-Juel1)179550 |b 4 |e Corresponding author |
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| 700 | 1 | _ | |a Sachse, Carsten |0 P:(DE-Juel1)173949 |b 10 |e Corresponding author |
| 773 | _ | _ | |a 10.1038/s41592-022-01586-0 |0 PERI:(DE-600)2163081-1 |p 38 |t Nature methods |v 1 |y 2022 |x 1548-7091 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/909655/files/s41592-022-01586-0.pdf |y OpenAccess |
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