000909837 001__ 909837
000909837 005__ 20221123131041.0
000909837 0247_ $$2Handle$$a2128/32733
000909837 0247_ $$2URN$$aurn:nbn:de:0001-2022112326
000909837 020__ $$a978-3-95806-642-7
000909837 037__ $$aFZJ-2022-03455
000909837 1001_ $$0P:(DE-Juel1)174340$$aFricke, Philipp Moritz$$b0$$eCorresponding author$$ufzj
000909837 245__ $$aEstablishing regulatable expression systems in the acetic acid bacterium Gluconobacter oxydans 621H$$f- 2022-11-23
000909837 260__ $$aJülich$$bForschungszentrum Jülich GmbH Zentralbibliothek, Verlag$$c2022
000909837 300__ $$aVIII, 187
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000909837 4900_ $$aSchriften des Forschungszentrums Jülich Reihe Schlüsseltechnologien / Key Technologies$$v258
000909837 502__ $$aDissertation, Univ. Düsseldorf, 2022$$bDissertation$$cUniv. Düsseldorf$$d2022
000909837 520__ $$aAmong industrially relevant microorganisms, the acetic acid bacterium Gluconobacter oxydans is valued for its ability to incompletely oxidize a vast number of carbohydrates stereoand regio-specifically. Biotechnological production processes involving G. oxydans strains so far utilized exclusively constitutive target gene expression. Regulatable promoters used in G. oxydans suffered from low induction fold-changes and relatively high basal promoter activity already when not induced. This study aimed to establish regulatable promoter systems in G. oxydans that allow tuned target gene expression in an effector-dependent manner. For this purpose, expression plasmids were constructed to test four well-characterized heterologous regulator-promoter pairs in G. oxydans. Additionally, screenings were performed to identify regulatable endogenous G. oxydans promoters responding to a metabolite given as an external stimulus and suitable for controlled gene expression.
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000909837 9141_ $$y2022
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