TY  - THES
AU  - Fricke, Philipp Moritz
TI  - Establishing regulatable expression systems in the acetic acid bacterium Gluconobacter oxydans 621H
VL  - 258
PB  - Univ. Düsseldorf
VL  - Dissertation
CY  - Jülich
M1  - FZJ-2022-03455
SN  - 978-3-95806-642-7
T2  - Schriften des Forschungszentrums Jülich Reihe Schlüsseltechnologien / Key Technologies
SP  - VIII, 187
PY  - 2022
N1  - Dissertation, Univ. Düsseldorf, 2022
AB  - Among industrially relevant microorganisms, the acetic acid bacterium Gluconobacter oxydans is valued for its ability to incompletely oxidize a vast number of carbohydrates stereoand regio-specifically. Biotechnological production processes involving G. oxydans strains so far utilized exclusively constitutive target gene expression. Regulatable promoters used in G. oxydans suffered from low induction fold-changes and relatively high basal promoter activity already when not induced. This study aimed to establish regulatable promoter systems in G. oxydans that allow tuned target gene expression in an effector-dependent manner. For this purpose, expression plasmids were constructed to test four well-characterized heterologous regulator-promoter pairs in G. oxydans. Additionally, screenings were performed to identify regulatable endogenous G. oxydans promoters responding to a metabolite given as an external stimulus and suitable for controlled gene expression.
LB  - PUB:(DE-HGF)3 ; PUB:(DE-HGF)11
UR  - https://juser.fz-juelich.de/record/909837
ER  -