TY  - JOUR
AU  - Wolff, Natalie
AU  - Kollenda, Sebastian
AU  - Klein, Kai
AU  - Loza, Kateryna
AU  - Heggen, Marc
AU  - Brochhagen, Leonie
AU  - Witzke, Oliver
AU  - Krawczyk, Adalbert
AU  - Hilger, Ingrid
AU  - Epple, Matthias
TI  - Silencing of proinflammatory NF-κB and inhibition of herpes simplex virus (HSV) replication by ultrasmall gold nanoparticles (2 nm) conjugated with small-interfering RNA
JO  - Nanoscale advances
VL  - 4
IS  - 21
SN  - 2516-0230
CY  - Cambridge
PB  - Royal Society of Chemistry
M1  - FZJ-2022-03551
SP  - 4502-4516 
PY  - 2022
AB  - Azide-terminated ultrasmall gold nanoparticles (2 nm gold core) were covalently functionalized with alkyne-terminated small-interfering siRNA duplexes by copper-catalyzed azide–alkyne cycloaddition (CuAAC; click chemistry). The nanoparticle core was visualized by transmission electron microscopy. The number of attached siRNA molecules per nanoparticle was determined by a combination of atomic absorption spectroscopy (AAS; for gold) and UV-Vis spectroscopy (for siRNA). Each nanoparticle carried between 6 and 10 siRNA duplex molecules which corresponds to a weight ratio of siRNA to gold of about 2.2 : 1. Different kinds of siRNA were conjugated to the nanoparticles, depending on the gene to be silenced. In general, the nanoparticles were readily taken up by cells and highly efficient in gene silencing, in contrast to free siRNA. This was demonstrated in HeLa-eGFP cells (silencing of eGFP) and in LPS-stimulated macrophages (silencing of NF-κB). Furthermore, we demonstrated that nanoparticles carrying antiviral siRNA potently inhibited the replication of Herpes simplex virus 2 (HSV-2) in vitro. This highlights the strong potential of siRNA-functionalized ultrasmall gold nanoparticles in a broad spectrum of applications, including gene silencing and treatment of viral infections, combined with a minimal dose of gold.
LB  - PUB:(DE-HGF)16
C6  - 36341304
UR  - <Go to ISI:>//WOS:000851568400001
DO  - DOI:10.1039/D2NA00250G
UR  - https://juser.fz-juelich.de/record/909961
ER  -