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@ARTICLE{Wolff:909961,
      author       = {Wolff, Natalie and Kollenda, Sebastian and Klein, Kai and
                      Loza, Kateryna and Heggen, Marc and Brochhagen, Leonie and
                      Witzke, Oliver and Krawczyk, Adalbert and Hilger, Ingrid and
                      Epple, Matthias},
      title        = {{S}ilencing of proinflammatory {NF}-κ{B} and inhibition of
                      herpes simplex virus ({HSV}) replication by ultrasmall gold
                      nanoparticles (2 nm) conjugated with small-interfering
                      {RNA}},
      journal      = {Nanoscale advances},
      volume       = {4},
      number       = {21},
      issn         = {2516-0230},
      address      = {Cambridge},
      publisher    = {Royal Society of Chemistry},
      reportid     = {FZJ-2022-03551},
      pages        = {4502-4516},
      year         = {2022},
      abstract     = {Azide-terminated ultrasmall gold nanoparticles (2 nm gold
                      core) were covalently functionalized with alkyne-terminated
                      small-interfering siRNA duplexes by copper-catalyzed
                      azide–alkyne cycloaddition (CuAAC; click chemistry). The
                      nanoparticle core was visualized by transmission electron
                      microscopy. The number of attached siRNA molecules per
                      nanoparticle was determined by a combination of atomic
                      absorption spectroscopy (AAS; for gold) and UV-Vis
                      spectroscopy (for siRNA). Each nanoparticle carried between
                      6 and 10 siRNA duplex molecules which corresponds to a
                      weight ratio of siRNA to gold of about 2.2 : 1.
                      Different kinds of siRNA were conjugated to the
                      nanoparticles, depending on the gene to be silenced. In
                      general, the nanoparticles were readily taken up by cells
                      and highly efficient in gene silencing, in contrast to free
                      siRNA. This was demonstrated in HeLa-eGFP cells (silencing
                      of eGFP) and in LPS-stimulated macrophages (silencing of
                      NF-κB). Furthermore, we demonstrated that nanoparticles
                      carrying antiviral siRNA potently inhibited the replication
                      of Herpes simplex virus 2 (HSV-2) in vitro. This highlights
                      the strong potential of siRNA-functionalized ultrasmall gold
                      nanoparticles in a broad spectrum of applications, including
                      gene silencing and treatment of viral infections, combined
                      with a minimal dose of gold.},
      cin          = {ER-C-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ER-C-1-20170209},
      pnm          = {5351 - Platform for Correlative, In Situ and Operando
                      Characterization (POF4-535)},
      pid          = {G:(DE-HGF)POF4-5351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {36341304},
      UT           = {WOS:000851568400001},
      doi          = {10.1039/D2NA00250G},
      url          = {https://juser.fz-juelich.de/record/909961},
}