000910210 001__ 910210
000910210 005__ 20221123131041.0
000910210 0247_ $$2Handle$$a2128/32735
000910210 0247_ $$2URN$$aurn:nbn:de:0001-2022112346
000910210 0247_ $$2URN$$aurn:nbn:de:0001-2022112346
000910210 020__ $$a978-3-95806-643-4
000910210 037__ $$aFZJ-2022-03687
000910210 1001_ $$0P:(DE-Juel1)169470$$aMeessen, Corinna$$b0$$eCorresponding author
000910210 245__ $$aHybrid hydrogels promote physiological functionality of long-term cultured primary neuronal cells in vitro$$f- 2022-11-23
000910210 260__ $$aJülich$$bForschungszentrum Jülich GmbH Zentralbibliothek, Verlag$$c2022
000910210 300__ $$ax, 120
000910210 3367_ $$2DataCite$$aOutput Types/Dissertation
000910210 3367_ $$0PUB:(DE-HGF)3$$2PUB:(DE-HGF)$$aBook$$mbook
000910210 3367_ $$2ORCID$$aDISSERTATION
000910210 3367_ $$2BibTeX$$aPHDTHESIS
000910210 3367_ $$02$$2EndNote$$aThesis
000910210 3367_ $$0PUB:(DE-HGF)11$$2PUB:(DE-HGF)$$aDissertation / PhD Thesis$$bphd$$mphd$$s1669187015_22943
000910210 3367_ $$2DRIVER$$adoctoralThesis
000910210 4900_ $$aSchriften des Forschungszentrums Jülich Reihe Information / Information$$v83
000910210 502__ $$aDissertation, RWTH Aachen, 2021$$bDissertation$$cRWTH Aachen$$d2021
000910210 520__ $$aThis study aimed to develop and establish a modular system for the 3D cultivation of primary neuronal cells, with the desirable tuneability in mechanical and chemical properties, while enhancing the cell viability for long-term studies. It could be shown that the novel Collagen-PVP-co-GMA3mol% biohybrid hydrogel promote physiological development of functional cortical networks. Primary cortical cells had been transfected with GCaMP6f to record spontaneous neuronal activity over 40 days in vitro. Network dynamics had been estimated based on generalizedtransfer entropy (GTE), showing the development of a functional network of balanced segregation and integration, with a strong emerging ability for resilience over 40 days in culture. Synchronous network activity could be observed already at day in vitro (DIV) 8-9 in absenceof external stimuli. Further, a correlation of network maturation as well as functional development with increasing molecular weight of PVP could be shown. With decreasing polymer chain length, neurite outgrowth and network maturation are significantly promoted. The addition ofPVP to collagen was demonstrated to not only support network functionality and growth, but also prevent the degradation of the hydrogel
000910210 536__ $$0G:(DE-HGF)POF4-899$$a899 - ohne Topic (POF4-899)$$cPOF4-899$$fPOF IV$$x0
000910210 8564_ $$uhttps://juser.fz-juelich.de/record/910210/files/Information_83.pdf$$yOpenAccess
000910210 909CO $$ooai:juser.fz-juelich.de:910210$$popenaire$$popen_access$$purn$$pdriver$$pVDB$$pdnbdelivery
000910210 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000910210 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000910210 9141_ $$y2022
000910210 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)169470$$aForschungszentrum Jülich$$b0$$kFZJ
000910210 9131_ $$0G:(DE-HGF)POF4-899$$1G:(DE-HGF)POF4-890$$2G:(DE-HGF)POF4-800$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0
000910210 920__ $$lyes
000910210 9201_ $$0I:(DE-Juel1)IBI-3-20200312$$kIBI-3$$lBioelektronik$$x0
000910210 980__ $$aphd
000910210 980__ $$aVDB
000910210 980__ $$aUNRESTRICTED
000910210 980__ $$abook
000910210 980__ $$aI:(DE-Juel1)IBI-3-20200312
000910210 9801_ $$aFullTexts