TY  - THES
AU  - Meessen, Corinna
TI  - Hybrid hydrogels promote physiological functionality of long-term cultured primary neuronal cells in vitro
VL  - 83
PB  - RWTH Aachen
VL  - Dissertation
CY  - Jülich
M1  - FZJ-2022-03687
SN  - 978-3-95806-643-4
T2  - Schriften des Forschungszentrums Jülich Reihe Information / Information
SP  - x, 120
PY  - 2022
N1  - Dissertation, RWTH Aachen, 2021
AB  - This study aimed to develop and establish a modular system for the 3D cultivation of primary neuronal cells, with the desirable tuneability in mechanical and chemical properties, while enhancing the cell viability for long-term studies. It could be shown that the novel Collagen-PVP-co-GMA3mol% biohybrid hydrogel promote physiological development of functional cortical networks. Primary cortical cells had been transfected with GCaMP6f to record spontaneous neuronal activity over 40 days in vitro. Network dynamics had been estimated based on generalizedtransfer entropy (GTE), showing the development of a functional network of balanced segregation and integration, with a strong emerging ability for resilience over 40 days in culture. Synchronous network activity could be observed already at day in vitro (DIV) 8-9 in absenceof external stimuli. Further, a correlation of network maturation as well as functional development with increasing molecular weight of PVP could be shown. With decreasing polymer chain length, neurite outgrowth and network maturation are significantly promoted. The addition ofPVP to collagen was demonstrated to not only support network functionality and growth, but also prevent the degradation of the hydrogel
LB  - PUB:(DE-HGF)3 ; PUB:(DE-HGF)11
UR  - https://juser.fz-juelich.de/record/910210
ER  -