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@PHDTHESIS{Meessen:910210,
      author       = {Meessen, Corinna},
      title        = {{H}ybrid hydrogels promote physiological functionality of
                      long-term cultured primary neuronal cells in vitro},
      volume       = {83},
      school       = {RWTH Aachen},
      type         = {Dissertation},
      address      = {Jülich},
      publisher    = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
      reportid     = {FZJ-2022-03687},
      isbn         = {978-3-95806-643-4},
      series       = {Schriften des Forschungszentrums Jülich Reihe Information
                      / Information},
      pages        = {x, 120},
      year         = {2022},
      note         = {Dissertation, RWTH Aachen, 2021},
      abstract     = {This study aimed to develop and establish a modular system
                      for the 3D cultivation of primary neuronal cells, with the
                      desirable tuneability in mechanical and chemical properties,
                      while enhancing the cell viability for long-term studies. It
                      could be shown that the novel $Collagen-PVP-co-GMA3mol\%$
                      biohybrid hydrogel promote physiological development of
                      functional cortical networks. Primary cortical cells had
                      been transfected with GCaMP6f to record spontaneous neuronal
                      activity over 40 days in vitro. Network dynamics had been
                      estimated based on generalizedtransfer entropy (GTE),
                      showing the development of a functional network of balanced
                      segregation and integration, with a strong emerging ability
                      for resilience over 40 days in culture. Synchronous network
                      activity could be observed already at day in vitro (DIV) 8-9
                      in absenceof external stimuli. Further, a correlation of
                      network maturation as well as functional development with
                      increasing molecular weight of PVP could be shown. With
                      decreasing polymer chain length, neurite outgrowth and
                      network maturation are significantly promoted. The addition
                      ofPVP to collagen was demonstrated to not only support
                      network functionality and growth, but also prevent the
                      degradation of the hydrogel},
      cin          = {IBI-3},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)3 / PUB:(DE-HGF)11},
      urn          = {urn:nbn:de:0001-2022112346},
      urn          = {urn:nbn:de:0001-2022112346},
      url          = {https://juser.fz-juelich.de/record/910210},
}