TY  - JOUR
AU  - Lübke, Nadine
AU  - Repges, Katharina
AU  - Menne, Christopher
AU  - Walker, Andreas
AU  - Jensen, Björn-Erik O.
AU  - Freise, Noemi F.
AU  - Gliga, Smaranda
AU  - Eickhoff, Simon
AU  - Bosse, Hans Martin
AU  - Adams, Ortwin
AU  - Timm, Jörg
TI  - Quantitative analysis of different respiratory specimens on two automated test systems for detection of SARS-CoV-2 RNA
JO  - Diagnostic microbiology and infectious disease
VL  - 105
IS  - 1
SN  - 0732-8893
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2022-03767
SP  - 115800 -
PY  - 2023
AB  - Molecular testing of SARS-CoV-2 RNA is essential during the pandemic. Here, we compared the results of different respiratory specimens including anterior nasal swabs, pharyngeal swabs, saliva swabs, and gargle lavage samples to nasopharyngeal swabs on two automated SARS-CoV-2 test systems. Samples were collected and tested simultaneously from a total of 36 hospitalized symptomatic COVID-19 patients. Detection and quantification of SARS-CoV-2 was performed on cobas®6800 (Roche) and NeuMoDx™ (Qiagen) systems. Both assays showed reliable detection and quantification of SARS-CoV-2 RNA, with nasopharyngeal swabs showing the highest sensitivity. SARS-CoV-2 RNA concentrations in other respiratory specimens were lower (mean 2.5 log10 copies/ml) or even undetectable in up to 20%. These data clearly indicate that not all respiratory materials are equally suitable for the management of hospitalized patients, especially, in the late phase of COVID-19, when the viral phase subsides and inflammation becomes the predominant factor, making detection of even lower viral loads increasingly important.
LB  - PUB:(DE-HGF)16
C6  - 36252283
UR  - <Go to ISI:>//WOS:000878990200001
DO  - DOI:10.1016/j.diagmicrobio.2022.115800
UR  - https://juser.fz-juelich.de/record/910348
ER  -