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@ARTICLE{Schenk:910684,
author = {Schenk, Janina and Höss, Sebastian and Kleinbölting, Nils
and Traunspurger, Walter},
title = {{S}uitability of molecular taxonomy for assessing polluted
sediments using the ${N}ema{SPEAR}[\%]$ index},
journal = {Ecological indicators},
volume = {137},
issn = {1470-160X},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {FZJ-2022-04056},
pages = {108761 -},
year = {2022},
abstract = {Assessing the ecological consequences of marine and
freshwater contamination is an important use of biological
indicators. The $NemaSPEAR[\%]$ index is a nematode-based
index for the evaluation of sediment quality and an
expedient complement to macroinvertebrate-based indicator
systems, especially for fine, cohesive sediments. While the
$NemaSPEAR[\%]$ index in its original form is
morphologically based, in this study the nematode
communities of 38 locations with known sediment
contamination were analyzed by light microscopy and
metabarcoding to demonstrate the validity of a
molecular-taxonomy-based $NemaSPEAR[\%]$ index. The results
showed that the molecular-based index can reliably
distinguish between sites of good, moderate, and bad
pollution status. Moreover, a greater congruence with
molecular read abundances were obtained after the
morphology-based $NemaSPEAR[\%]$ index was corrected for
differences in the biomass of different nematode species.
However, incomplete reference databases hamper a full
congruence between the species inventories recorded in
morphological and molecular datasets. Another source of
variability is the substantial difference in the biomass
(and thus also of gene copies) of different species, thus
evidencing a bias in abundance-based calculations of the
$NemaSPEAR[\%]$ index. Despite these limitations, the
molecular approach provides an expertise-free means of
reliable bioindication using one of the most abundant and
diverse components of benthic macroinvertebrate communities.
Moreover, due to the ubiquity of nematodes, application of
the index can be extended to wastewater, biofilm, and other
lotic and lentic waters that require regular monitoring.},
cin = {IBG-5},
ddc = {630},
cid = {I:(DE-Juel1)IBG-5-20220217},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000778350200003},
doi = {10.1016/j.ecolind.2022.108761},
url = {https://juser.fz-juelich.de/record/910684},
}