Gast ::
| Hauptseite > Publikationsdatenbank > Enzymatic C3-Methylation of Indoles Using Methyltransferase PsmD─Crystal Structure, Catalytic Mechanism, and Preparative Applications > print |
| Hauptseite > Publikationsdatenbank > Enzymatic C3-Methylation of Indoles Using Methyltransferase PsmD─Crystal Structure, Catalytic Mechanism, and Preparative Applications > print |
| 001 | 911150 | ||
| 005 | 20230224084258.0 | ||
| 024 | 7 | _ | |a 10.1021/acscatal.2c04240 |2 doi |
| 024 | 7 | _ | |a WOS:000881780200001 |2 WOS |
| 037 | _ | _ | |a FZJ-2022-04465 |
| 082 | _ | _ | |a 540 |
| 100 | 1 | _ | |a Amariei, Diana A. |0 P:(DE-Juel1)185993 |b 0 |
| 245 | _ | _ | |a Enzymatic C3-Methylation of Indoles Using Methyltransferase PsmD─Crystal Structure, Catalytic Mechanism, and Preparative Applications |
| 260 | _ | _ | |a Washington, DC |c 2022 |b ACS |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1674463077_17617 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Enantioselective methylation is a challenging task in organic chemistry, yet often desirable in drug discovery and optimization. S-Adenosyl methionine (SAM)-dependent methyltransferases (MTases) offer a selective alternative to chemical synthesis and an abundance of potential scaffolds. The crystal structure of C3-indole MTase PsmD from Streptomyces griseofuscus, involved in the biosynthesis of the acetylcholinesterase inhibitor physostigmine, was determined via X-ray crystallography. The amino acid residues essential for catalysis were identified by site-directed mutagenesis, and a mechanism of action was proposed. Furthermore, a PsmD ortholog was identified and characterized. The variant catalyzed enantioselective C-methylation over a broad substrate scope while displaying increased stability. Using this enzyme, preparative-scale enzymatic methylation was performed in cell-free extracts in combination with an SAM recycling system, eliminating the need for cofactor supplementation. |
| 536 | _ | _ | |a 2171 - Biological and environmental resources for sustainable use (POF4-217) |0 G:(DE-HGF)POF4-2171 |c POF4-217 |f POF IV |x 0 |
| 536 | _ | _ | |a 5241 - Molecular Information Processing in Cellular Systems (POF4-524) |0 G:(DE-HGF)POF4-5241 |c POF4-524 |f POF IV |x 1 |
| 588 | _ | _ | |a Dataset connected to CrossRef, Journals: juser.fz-juelich.de |
| 700 | 1 | _ | |a Pozhydaieva, Nadiia |0 P:(DE-Juel1)180818 |b 1 |
| 700 | 1 | _ | |a David, Benoit |0 P:(DE-Juel1)190107 |b 2 |
| 700 | 1 | _ | |a Schneider, Pascal |0 P:(DE-Juel1)176354 |b 3 |
| 700 | 1 | _ | |a Classen, Thomas |0 P:(DE-Juel1)128890 |b 4 |
| 700 | 1 | _ | |a Gohlke, Holger |0 P:(DE-Juel1)172663 |b 5 |
| 700 | 1 | _ | |a Weiergräber, Oliver H. |0 P:(DE-Juel1)131988 |b 6 |
| 700 | 1 | _ | |a Pietruszka, Jörg |0 P:(DE-Juel1)128906 |b 7 |e Corresponding author |
| 773 | _ | _ | |a 10.1021/acscatal.2c04240 |g p. 14130 - 14139 |0 PERI:(DE-600)2584887-2 |n 22 |p 14130 - 14139 |t ACS catalysis |v 12 |y 2022 |x 2155-5435 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/911150/files/acscatal.2c04240.pdf |y Restricted |
| 909 | C | O | |p VDB |o oai:juser.fz-juelich.de:911150 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 0 |6 P:(DE-Juel1)185993 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 2 |6 P:(DE-Juel1)190107 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)176354 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 4 |6 P:(DE-Juel1)128890 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 5 |6 P:(DE-Juel1)172663 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 6 |6 P:(DE-Juel1)131988 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 7 |6 P:(DE-Juel1)128906 |
| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Erde und Umwelt |l Erde im Wandel – Unsere Zukunft nachhaltig gestalten |1 G:(DE-HGF)POF4-210 |0 G:(DE-HGF)POF4-217 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-200 |4 G:(DE-HGF)POF |v Für eine nachhaltige Bio-Ökonomie – von Ressourcen zu Produkten |9 G:(DE-HGF)POF4-2171 |x 0 |
| 913 | 1 | _ | |a DE-HGF |b Key Technologies |l Natural, Artificial and Cognitive Information Processing |1 G:(DE-HGF)POF4-520 |0 G:(DE-HGF)POF4-524 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-500 |4 G:(DE-HGF)POF |v Molecular and Cellular Information Processing |9 G:(DE-HGF)POF4-5241 |x 1 |
| 914 | 1 | _ | |y 2022 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b ACS CATAL : 2021 |d 2022-11-15 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2022-11-15 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2022-11-15 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2022-11-15 |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0113 |2 StatID |b Science Citation Index Expanded |d 2022-11-15 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2022-11-15 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1150 |2 StatID |b Current Contents - Physical, Chemical and Earth Sciences |d 2022-11-15 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0160 |2 StatID |b Essential Science Indicators |d 2022-11-15 |
| 915 | _ | _ | |a IF >= 10 |0 StatID:(DE-HGF)9910 |2 StatID |b ACS CATAL : 2021 |d 2022-11-15 |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)IBG-4-20200403 |k IBG-4 |l Bioinformatik |x 0 |
| 920 | 1 | _ | |0 I:(DE-Juel1)IBI-7-20200312 |k IBI-7 |l Strukturbiochemie |x 1 |
| 920 | 1 | _ | |0 I:(DE-Juel1)IBOC-20090406 |k IBOC |l Institut für Bioorganische Chemie (HHUD) |x 2 |
| 920 | 1 | _ | |0 I:(DE-Juel1)IBG-1-20101118 |k IBG-1 |l Biotechnologie |x 3 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-Juel1)IBG-4-20200403 |
| 980 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| 980 | _ | _ | |a I:(DE-Juel1)IBOC-20090406 |
| 980 | _ | _ | |a I:(DE-Juel1)IBG-1-20101118 |
| 980 | _ | _ | |a UNRESTRICTED |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|