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@ARTICLE{Paulikat:912325,
author = {Paulikat, Mirko and Aranda, Juan and Ippoliti, Emiliano and
Orozco, Modesto and Carloni, Paolo},
title = {{P}roton {T}ransfers to {DNA} in {N}ative {E}lectrospray
{I}onization {M}ass {S}pectrometry: {A} {Q}uantum
{M}echanics/{M}olecular {M}echanics {S}tudy},
journal = {The journal of physical chemistry letters},
volume = {13},
issn = {1948-7185},
address = {Washington, DC},
publisher = {ACS},
reportid = {FZJ-2022-05517},
pages = {12004 - 12010},
year = {2022},
abstract = {Native electrospray ionization-ion mobility mass
spectrometry (N-ESI/IM-MS) is a powerful approach for
low-resolution structural studies of DNAs in the free state
and in complex with ligands. Solvent vaporization is coupled
with proton transfers from ammonium ions to the DNA,
resulting in a reduction of the DNA charge. Here we provide
insight into these processes by classical molecular dynamics
and quantum mechanics/molecular mechanics free energy
calculations on the d(GpCpGpApApGpC) heptamer, for which a
wealth of experiments is available. Our multiscale
simulations, consistent with experimental data, reveal a
highly complex scenario. The proton either sits on one of
the molecules or is fully delocalized on both, depending on
the level of hydration of the analytes and the size of the
droplets formed during the electrospray experiments. This
work complements our previous study of the intramolecular
proton transfer on the same heptamer occurring after the
processes studied here, and together, they provide a first
molecular view of proton transfer in N-ESI/IM-MS.},
cin = {IAS-5 / INM-9},
ddc = {530},
cid = {I:(DE-Juel1)IAS-5-20120330 / I:(DE-Juel1)INM-9-20140121},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {36540944},
UT = {WOS:000904441400001},
doi = {10.1021/acs.jpclett.2c03100},
url = {https://juser.fz-juelich.de/record/912325},
}