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@ARTICLE{Hansen:915920,
author = {Hansen, Jan and Pedersen, Jannik N. and Pedersen, Jan Skov
and Egelhaaf, Stefan U. and Platten, Florian},
title = {{U}niversal effective interactions of globular proteins
close to liquid–liquid phase separation:
{C}orresponding-states behavior reflected in the structure
factor},
journal = {The journal of chemical physics},
volume = {156},
number = {24},
issn = {0021-9606},
address = {Melville, NY},
publisher = {American Institute of Physics},
reportid = {FZJ-2022-05783},
pages = {244903 -},
year = {2022},
abstract = {Intermolecular interactions in protein solutions, in
general, contain many contributions. If short-range
attractions dominate, the state diagram exhibits
liquid–liquid phase separation (LLPS) that is metastable
with respect to crystallization. In this case, the extended
law of corresponding states (ELCS) suggests that
thermodynamic properties are insensitive to details of the
underlying interaction potential. Using lysozyme solutions,
we investigate the applicability of the ELCS to the static
structure factor and how far effective colloidal interaction
models can help to rationalize the phase behavior and
interactions of protein solutions in the vicinity of the
LLPS binodal. The (effective) structure factor has been
determined by small-angle x-ray scattering. It can be
described by Baxter’s adhesive hard-sphere model, which
implies a single fit parameter from which the normalized
second virial coefficient b2 is inferred and found to
quantitatively agree with previous results from static light
scattering. The b2 values are independent of protein
concentration but systematically vary with temperature and
solution composition, i.e., salt and additive content. If
plotted as a function of temperature normalized by the
critical temperature, the values of b2 follow a universal
behavior. These findings validate the applicability of the
ELCS to globular protein solutions and indicate that the
ELCS can also be reflected in the structure factor.},
cin = {IBI-4},
ddc = {530},
cid = {I:(DE-Juel1)IBI-4-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {35778071},
UT = {WOS:000816964600002},
doi = {10.1063/5.0088601},
url = {https://juser.fz-juelich.de/record/915920},
}