TY  - JOUR
AU  - Pantoja, Christian
AU  - Zweckstetter, Markus
AU  - Rezaie Ghaleh, Nasrollah
TI  - Dynamical component exchange in a model phase separating system: an NMR-based approach
JO  - Physical chemistry, chemical physics
VL  - 24
IS  - 10
SN  - 1463-9076
CY  - Cambridge
PB  - RSC Publ.
M1  - FZJ-2022-05896
SP  - 6169 - 6175
PY  - 2022
AB  - Biomolecular phase separation plays a key role in the spatial organization of cellular activities. Dynamic formation and rapid component exchange between phase separated cellular bodies and their environment are crucial for their function. Here, we employ a well-established phase separating model system, namely, a triethylamine (TEA)–water mixture, and develop an NMR approach to detect the exchange of scaffolding TEA molecules between separate phases and determine the underlying exchange rate. We further demonstrate how the advantageous NMR properties of fluorine nuclei provide access to otherwise inaccessible exchange processes of a client molecule. The developed NMR-based approach allows quantitative monitoring of the effect of regulatory factors on component exchange and facilitates “exchange”-based screening and optimization of small molecules against druggable biomolecular targets located inside condensed phases.
LB  - PUB:(DE-HGF)16
C6  - 35229098
UR  - <Go to ISI:>//WOS:000762379400001
DO  - DOI:10.1039/D2CP00042C
UR  - https://juser.fz-juelich.de/record/916050
ER  -