000917018 001__ 917018
000917018 005__ 20231027114351.0
000917018 0247_ $$2doi$$a10.1016/j.biochi.2022.11.014
000917018 0247_ $$2ISSN$$a0300-9084
000917018 0247_ $$2ISSN$$a1638-6183
000917018 0247_ $$2Handle$$a2128/34034
000917018 0247_ $$2pmid$$a36460205
000917018 0247_ $$2WOS$$aWOS:000943144700001
000917018 037__ $$aFZJ-2023-00284
000917018 082__ $$a540
000917018 1001_ $$00000-0002-8672-4514$$aCombet, Sophie$$b0$$eCorresponding author
000917018 245__ $$aEffect of amphiphilic environment on the solution structure of mouse TSPO translocator protein
000917018 260__ $$aParis [u.a.]$$bElsevier$$c2023
000917018 3367_ $$2DRIVER$$aarticle
000917018 3367_ $$2DataCite$$aOutput Types/Journal article
000917018 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1677569129_13022
000917018 3367_ $$2BibTeX$$aARTICLE
000917018 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000917018 3367_ $$00$$2EndNote$$aJournal Article
000917018 520__ $$aThe translocator protein (TSPO) is a ubiquitous transmembrane protein of great pharmacological interest thanks to its high affinity to many drug ligands. The only high-resolution 3D-structure known for mammalian TSPO was obtained by NMR for the mouse mTSPO in DPC detergent only in presence of the high-affinity PK 11195 ligand. An atomic structure of free-ligand mTSPO is still missing to better understand the interaction of ligands with mTSPO and their effects on the protein conformation.Here, we decipher the solution structures of the recombinant mTSPO without ligand both in (i) SDS, the detergent used to extract and purify the protein from E. coli inclusion bodies, and (ii) DPC, the detergent used to solve the PK 11195-binding mTSPO NMR structure.We report partially refolded and less flexible mTSPO helices in DPC compared to SDS. Besides, DPC stabilizes the tertiary structure of mTSPO, as shown by a higher intrinsic Trp fluorescence and changes in indole environment.We evaluate by SEC-MALLS that ∼135 SDS and ∼100 DPC molecules are bound to mTSPO. SEC-small-angle X-ray (SAXS) and neutron (SANS) scattering confirm a larger mTSPO-detergent complex in SDS than in DPC. Using the contrast-matching technique in SEC-SANS, we demonstrate that mTSPO conformation is more compact and less flexible in DPC than in SDS. Combining ab initio modeling with SANS, we confirm that mTSPO conformation is less elongated in DPC than in SDS. However, the free-ligand mTSPO envelope in DPC is not as compact as the PK 11195-binding protein NMR structure, the ligand stiffening the protein.
000917018 536__ $$0G:(DE-HGF)POF4-6G4$$a6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ) (POF4-6G4)$$cPOF4-6G4$$fPOF IV$$x0
000917018 536__ $$0G:(DE-HGF)POF4-632$$a632 - Materials – Quantum, Complex and Functional Materials (POF4-632)$$cPOF4-632$$fPOF IV$$x1
000917018 588__ $$aDataset connected to CrossRef, Journals: juser.fz-juelich.de
000917018 65027 $$0V:(DE-MLZ)SciArea-160$$2V:(DE-HGF)$$aBiology$$x0
000917018 65017 $$0V:(DE-MLZ)GC-130-2016$$2V:(DE-HGF)$$aHealth and Life$$x0
000917018 693__ $$0EXP:(DE-MLZ)NOSPEC-20140101$$5EXP:(DE-MLZ)NOSPEC-20140101$$eNo specific instrument$$x0
000917018 7001_ $$0P:(DE-HGF)0$$aBonneté, Françoise$$b1$$eCorresponding author
000917018 7001_ $$aFinet, Stéphanie$$b2
000917018 7001_ $$aPozza, Alexandre$$b3
000917018 7001_ $$aSaade, Christelle$$b4
000917018 7001_ $$aMartel, Anne$$b5
000917018 7001_ $$0P:(DE-Juel1)158075$$aKoutsioumpas, Alexandros$$b6$$ufzj
000917018 7001_ $$aLacapère, Jean-Jacques$$b7
000917018 773__ $$0PERI:(DE-600)2011725-5$$a10.1016/j.biochi.2022.11.014$$gp. S0300908422003121$$p61-72$$tBiochimie$$v205$$x0300-9084$$y2023
000917018 8564_ $$uhttps://juser.fz-juelich.de/record/917018/files/20221122_mTSPO_main.pdf$$yPublished on 2022-11-29. Available in OpenAccess from 2023-11-29.
000917018 909CO $$ooai:juser.fz-juelich.de:917018$$pdnbdelivery$$pVDB$$pVDB:MLZ$$pdriver$$popen_access$$popenaire
000917018 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)158075$$aForschungszentrum Jülich$$b6$$kFZJ
000917018 9131_ $$0G:(DE-HGF)POF4-6G4$$1G:(DE-HGF)POF4-6G0$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lGroßgeräte: Materie$$vJülich Centre for Neutron Research (JCNS) (FZJ)$$x0
000917018 9131_ $$0G:(DE-HGF)POF4-632$$1G:(DE-HGF)POF4-630$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vMaterials – Quantum, Complex and Functional Materials$$x1
000917018 9141_ $$y2023
000917018 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2022-11-23
000917018 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2022-11-23
000917018 915__ $$0LIC:(DE-HGF)CCBYNCND4$$2HGFVOC$$aCreative Commons Attribution-NonCommercial-NoDerivs CC BY-NC-ND 4.0
000917018 915__ $$0StatID:(DE-HGF)0530$$2StatID$$aEmbargoed OpenAccess
000917018 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2022-11-23
000917018 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz$$d2023-10-22$$wger
000917018 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2023-10-22
000917018 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2023-10-22
000917018 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2023-10-22
000917018 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2023-10-22
000917018 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2023-10-22
000917018 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bBIOCHIMIE : 2022$$d2023-10-22
000917018 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2023-10-22
000917018 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2023-10-22
000917018 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2023-10-22
000917018 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5$$d2023-10-22
000917018 920__ $$lyes
000917018 9201_ $$0I:(DE-Juel1)JCNS-FRM-II-20110218$$kJCNS-FRM-II$$lJCNS-FRM-II$$x0
000917018 9201_ $$0I:(DE-588b)4597118-3$$kMLZ$$lHeinz Maier-Leibnitz Zentrum$$x1
000917018 9201_ $$0I:(DE-Juel1)JCNS-1-20110106$$kJCNS-1$$lNeutronenstreuung$$x2
000917018 9201_ $$0I:(DE-Juel1)JCNS-4-20201012$$kJCNS-4$$lJCNS-4$$x3
000917018 980__ $$ajournal
000917018 980__ $$aVDB
000917018 980__ $$aUNRESTRICTED
000917018 980__ $$aI:(DE-Juel1)JCNS-FRM-II-20110218
000917018 980__ $$aI:(DE-588b)4597118-3
000917018 980__ $$aI:(DE-Juel1)JCNS-1-20110106
000917018 980__ $$aI:(DE-Juel1)JCNS-4-20201012
000917018 9801_ $$aFullTexts