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@ARTICLE{Esch:917120,
      author       = {Esch, Lara and Kirsch, Christian and Vogel, Lara and Kelm,
                      Jana and Huwa, Nikolai and Schmitz, Maike and Classen,
                      Thomas and Schaffrath, Ulrich},
      title        = {{P}athogen {R}esistance {D}epending on {J}acalin-{D}irigent
                      {C}himeric {P}roteins {I}s {C}ommon among {P}oaceae but
                      {A}bsent in the {D}icot {A}rabidopsis as {E}videnced by
                      {A}nalysis of {H}omologous {S}ingle-{D}omain {P}roteins},
      journal      = {Plants},
      volume       = {12},
      number       = {1},
      issn         = {2223-7747},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2023-00352},
      pages        = {67 -},
      year         = {2023},
      abstract     = {MonocotJRLs are Poaceae-specific two-domain proteins that
                      consist of a jacalin-related lectin (JRL) and a dirigent
                      (DIR) domain which participate in multiple developmental
                      processes, including disease resistance. For OsJAC1, a
                      monocotJRL from rice, it has been confirmed that
                      constitutive expression in transgenic rice or barley plants
                      facilitates broad-spectrum disease resistance. In this
                      process, both domains of OsJAC1 act cooperatively, as
                      evidenced from experiments with artificially separated JRL-
                      or DIR-domain-containing proteins. Interestingly, these
                      chimeric proteins did not evolve in dicotyledonous plants.
                      Instead, proteins with a single JRL domain, multiple JRL
                      domains or JRL domains fused to domains other than DIR
                      domains are present. In this study, we wanted to test if the
                      cooperative function of JRL and DIR proteins leading to
                      pathogen resistance was conserved in the dicotyledonous
                      plant Arabidopsis thaliana. In Arabidopsis, we identified 50
                      JRL and 24 DIR proteins, respectively, from which seven
                      single-domain JRL and two single-domain DIR candidates were
                      selected. A single-cell transient gene expression assay in
                      barley revealed that specific combinations of the
                      Arabidopsis JRL and DIR candidates reduced the penetration
                      success of barley powdery mildew. Strikingly, one of these
                      pairs, AtJAX1 and AtDIR19, is encoded by genes located next
                      to each other on chromosome one. However, when using natural
                      variation and analyzing Arabidopsis ecotypes that express
                      full-length or truncated versions of AtJAX1, the
                      presence/absence of the full-length AtJAX1 protein could not
                      be correlated with resistance to the powdery mildew fungus
                      Golovinomyces orontii. Furthermore, an analysis of the
                      additional JRL and DIR candidates in a bi-fluorescence
                      complementation assay in Nicotiana benthamiana revealed no
                      direct interaction of these JRL/DIR pairs. Since transgenic
                      Arabidopsis plants expressing OsJAC1-GFP also did not show
                      increased resistance to G. orontii, it was concluded that
                      the resistance mediated by the synergistic activities of DIR
                      and JRL proteins is specific for members of the Poaceae, at
                      least regarding the resistance against powdery mildew.
                      Arabidopsis lacks the essential components of the
                      DIR-JRL-dependent resistance pathway.},
      cin          = {IBOC / IBG-1},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBOC-20090406 / I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217) / DFG
                      project 369034981 - Mechanismen der durch
                      Dirigent/Jacalin-Proteinpaare hervorgerufenen
                      Breitspektrumresistenz in Pflanzen gegen pilzliche
                      Pathogene},
      pid          = {G:(DE-HGF)POF4-2172 / G:(GEPRIS)369034981},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {36616196},
      UT           = {WOS:000909603800001},
      doi          = {10.3390/plants12010067},
      url          = {https://juser.fz-juelich.de/record/917120},
}