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@INPROCEEDINGS{Schultes:917381,
      author       = {Schultes, S and Hinz, Carsten and Metzner, Ralf and
                      Pflugfelder, Daniel and Chlubek, Antonia and van Dusschoten,
                      Dagmar and Huber, Gregor and Bauke, S and Watt, M and
                      Koller, Robert and Knief, C},
      title        = {{C}ombining an 11{C} and 13 {C} labelling approach to track
                      photosynthates into the root system andrhizosphere
                      microbiota of maize},
      reportid     = {FZJ-2023-00595},
      year         = {2022},
      abstract     = {Plants transport substantial amounts of fresh
                      photosynthates into their root system. From there, the
                      photosynthates are par-tially released into the rhizosphere
                      by rhizodeposition, which promotes interactions with
                      microorganisms. Little is known aboutthe spatial
                      distribution of photosynthates within the root system, and
                      how these distributional patterns relate to
                      rhizodepositionand the assembly of rhizosphere microbial
                      communities. To assess root-internal photosynthate
                      distribution, we labelled youngmaize plants with short-lived
                      11CO2 on days 14 and 21 after sowing. 11C allocation within
                      the root system was then visualized usingnon-invasive
                      positron emission tomography (PET) in combination with
                      magnetic resonance imaging (MRI). The same plants
                      werelabelled with the stable isotope 13CO2 for 6 consecutive
                      days in order to trace the path of photosynthates into the
                      rhizosphereand microbiota. Roots and rhizosphere soil were
                      harvested on day 22, using the previously acquired PET/MRI
                      scans to target rootregions with distinct photosynthate
                      levels. Rhizosphere organisms that consumed 13C-labelled
                      photosynthates were identifiedby DNA stable isotope probing
                      followed by amplicon sequencing. Isotope ratio mass
                      spectrometry (IRMS) was used to quantify13C in rhizosphere
                      soil. The visualization and quantification of root-internal
                      11C by PET combined with MRI revealed an
                      increasedaccumulation of photosynthates in root tips,
                      particularly in young crown root tips. This pattern was
                      partially reflected in thedistribution of 13C in the
                      rhizosphere as determined by IRMS. Previous results of
                      PET/MRI guided rhizosphere sampling alreadyrevealed a slight
                      effect of photosynthate distribution within the root system
                      on the local microbial community structure. Now,this effect
                      is being tested with a more sensitive method that focuses on
                      the direct microbial consumers of photosynthates bycombining
                      11CO2 labelling with 13C stable isotope probing. During the
                      conference, the results of microbial community analysis
                      willbe discussed, along with the associated root-internal
                      and -external photosynthate allocation.},
      month         = {Aug},
      date          = {2022-08-29},
      organization  = {Botanik-Tagung International
                       Conference of the German Society for
                       Plant Sciences 2022, Bonn (Germany), 29
                       Aug 2022 - 2 Sep 2022},
      subtyp        = {After Call},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/917381},
}