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| Home > Publications database > Allocation patterns of recently fixed carbon in the root system of maize influence the rhizosphere microbiota |
| Home > Publications database > Allocation patterns of recently fixed carbon in the root system of maize influence the rhizosphere microbiota |
| Conference Presentation (After Call) | FZJ-2023-00596 |
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2022
Abstract: Plants transport substantial amounts of recently fixed carbon into their root system. This carbon is partially released into the rhizosphere by rhizodeposition, where it supports life of rhizosphere microorganisms. Little is known about the spatial distribution of recently fixed C within the root system, and how these distributional patterns relate to rhizodeposition and the assembly and activity of rhizosphere microbial communities. To assess root-internal C distribution, we labelled maize plants with short-lived 11CO2. 11C allocation within the root system was visualized using positron emission tomography (PET) in combination with magnetic resonance imaging (MRI). Image-guided sampling of the rhizosphere was then performed on day 21 after sowing from different root types and “tracer rich” versus “tracer poor” root regions to reveal local differences in the rhizosphere microbiota. Rhizosphere samples were analysed based on amplicon sequencing targeting bacteria and fungi. This revealed that root type caused spatial variation in the rhizosphere microbiota, while C distribution in the root system influenced the community structure rather slightly. To further analyse the link between root-internal carbon allocation patterns and carbon metabolism in the rhizosphere, 11CO2 labelling was combined with 13CO2 labelling. Isotope ratio mass spectrometry (IRMS) analyses of rhizosphere soil showed that root-internal carbon allocation patterns were mostly well reflected in the distribution of 13C in the rhizosphere soil. The metabolically active rhizosphere organisms were identified based on DNA stable isotope probing. The results of this spatially resolved community analyses along with the associated root-internal C allocation patterns and 13C-patterns in the rhizosphere will be discussed in the conference presentation.
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