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@MASTERSTHESIS{ReineckeLevi:917401,
author = {Reinecke-Levi, Diana and Große, David and Janzik, Ingar},
title = {{C}ulture optimization and characterization of three
{P}orphyridium strains for valuable compound production},
school = {FH Aachen},
type = {Bachelorarbeit},
reportid = {FZJ-2023-00615},
pages = {22},
year = {2022},
note = {Bachelorarbeit, FH Aachen, 2022},
abstract = {The optimal conditions to maximize phycoerythrin- (PE) and
exopolysaccharide (EPS) production in the three
photoautotrophic red marine microalgal strains of
Porphyridium purpureum (SAG 1380-1a/-1d) and cruentum (UTEX
161) were researched. Design of experiment software (DoE)
was used in planning and evaluation. Dependencies were found
between light intensity, nitrogen and magnesium
concentrations and the PE and EPS productivities. The
maximal PE productivity (4,96 µg mL-1d-1) was found in P.
purpureum SAG 1380-1d. Contrasting, the highest EPS
productivity (5,75 g l-1 d-1) was found in P. purpureum SAG
1380-1a. However, optimization efforts in EPS production
remained low due to low dependencies under tested growth
conditions. The optimal product inducing conditions for all
strains were identified at ~40 µmol photon m-2 s-1 and 9-15
mM nitrogen. The optimal magnesium concentration ranged
between 5, 38 and 45 mM for UTEX 161, SAG 1380-1a and -1d,
respectively. The pilot scale cultivation under greenhouse
conditions could not be finished, because of
temperature-stress during the cultivation.},
cin = {IBG-2},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {2171 - Biological and environmental resources for
sustainable use (POF4-217)},
pid = {G:(DE-HGF)POF4-2171},
typ = {PUB:(DE-HGF)2},
url = {https://juser.fz-juelich.de/record/917401},
}