TY  - JOUR
AU  - Jansen, R.M.C.
AU  - Miebach, M.
AU  - Kleist, E.
AU  - van Henten, E. J.
AU  - Wildt, J.
TI  - Release of lipoxygenase products and monoterpenes by tomato plants as an indicator of Botrytis cinerea-induced stress
JO  - Plant biology
VL  - 11
SN  - 1435-8603
CY  - Oxford [u.a.] :Wiley- Blackwell
PB  - Wiley-Blackwell - STM
M1  - PreJuSER-923
SP  - 859 - 868
PY  - 2009
N1  - We thank Dr Jan van Kan for providing the Botrytis cinerea strain and for helpful comments on the manuscript. This research received grant-aided support from the European Science Foundation Scientific Programme 'Volatile organic compounds in the Biosphere-Atmosphere System' (VOCBAS).
AB  - Changes in emission of volatile organic compounds (VOCs) from tomato induced by the fungus Botrytis cinerea were studied in plants inoculated by spraying with suspensions containing B. cinerea spores. VOC emissions were analysed using on-line gas chromatography-mass spectrometry, with a time resolution of about 1 h, for up to 2 days after spraying. Four phases were delimited according to the starting point and the applied day/night rhythm of the experiments. These phases were used to demonstrate changes in VOC flux caused by B. cinerea infestation. Tomato plants inoculated with B. cinerea emitted a different number and amount of VOCs after inoculation compared to control plants that had been sprayed with a suspension without B. cinerea spores. The changes in emissions were dependent on time after inoculation as well as on the severity of infection. The predominant VOCs emitted after inoculation were volatile products from the lipoxygenase pathway (LOX products). The increased emission of LOX products proved to be a strong indicator of a stress response, indicating that VOC emissions can be used to detect plant stress at an early stage. Besides emission of LOX products, there were also increases in monoterpene emissions. However, neither increased emission of LOX products nor of monoterpenes is specific for B. cinerea attack. The emission of LOX products is also induced by other stresses, and increased emission of monoterpenes seems to be the result of mechanical damage induced by secondary stress impacts on leaves.
KW  - Botrytis: physiology
KW  - Gas Chromatography-Mass Spectrometry
KW  - Lipoxygenase: metabolism
KW  - Lycopersicon esculentum: enzymology
KW  - Lycopersicon esculentum: metabolism
KW  - Lycopersicon esculentum: microbiology
KW  - Monoterpenes: metabolism
KW  - Plant Leaves: enzymology
KW  - Plant Leaves: metabolism
KW  - Plant Leaves: microbiology
KW  - Plant Proteins: metabolism
KW  - Stress, Physiological
KW  - Time Factors
KW  - Volatile Organic Compounds: metabolism
KW  - Monoterpenes (NLM Chemicals)
KW  - Plant Proteins (NLM Chemicals)
KW  - Volatile Organic Compounds (NLM Chemicals)
KW  - Lipoxygenase (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19796363
UR  - <Go to ISI:>//WOS:000270429300009
DO  - DOI:10.1111/j.1438-8677.2008.00183.x
UR  - https://juser.fz-juelich.de/record/923
ER  -