%0 Journal Article
%A Marjault, Henri-Baptiste
%A Yang-Sung, Sohn
%A Zuo, Ke
%A Carloni, Paolo
%A Mittler, Ron
%A Nechushtai, Rachel
%T Structure-Based Screening Reveals a Ligand That Stabilizes the [2Fe-2S] Clusters of Human mitoNEET and Reduces Ovarian Cancer Cell Proliferation
%J The journal of physical chemistry  / B
%V 126
%N 46
%@ 1520-6106
%C Washington, DC
%I Soc.
%M FZJ-2023-00977
%P 9559 - 9565
%D 2022
%X Human NEET proteins play an important role in a variety of diseases, including cancer. Using the recently published X-ray structure of the human mNT-M1 complex, we screened a commercial chemical compound library and identified a new human mitoNEET (mNT) binding ligand (NTS-01). Biochemical investigations revealed that NTS-01 specifically binds to the human mNT protein and stabilizes its [2Fe-2S] clusters under oxidative conditions in vitro. Treatment of ovarian cancer cells with NTS-01 induces ovarian cancer (SKOV-3) mitochondrial fragmentation (fission) and reduces ovarian cancer cell proliferation in a 2D single-layer cell culture, as well as in a 3D-spheroids culture. The NTS-01 molecule represents therefore a new lead compound for further drug design studies attempting to develop efficient treatment against ovarian cancer.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 36374279
%U <Go to ISI:>//WOS:000885419200001
%R 10.1021/acs.jpcb.2c05728
%U https://juser.fz-juelich.de/record/943378