TY  - JOUR
AU  - Marjault, Henri-Baptiste
AU  - Yang-Sung, Sohn
AU  - Zuo, Ke
AU  - Carloni, Paolo
AU  - Mittler, Ron
AU  - Nechushtai, Rachel
TI  - Structure-Based Screening Reveals a Ligand That Stabilizes the [2Fe-2S] Clusters of Human mitoNEET and Reduces Ovarian Cancer Cell Proliferation
JO  - The journal of physical chemistry  / B
VL  - 126
IS  - 46
SN  - 1520-6106
CY  - Washington, DC
PB  - Soc.
M1  - FZJ-2023-00977
SP  - 9559 - 9565
PY  - 2022
AB  - Human NEET proteins play an important role in a variety of diseases, including cancer. Using the recently published X-ray structure of the human mNT-M1 complex, we screened a commercial chemical compound library and identified a new human mitoNEET (mNT) binding ligand (NTS-01). Biochemical investigations revealed that NTS-01 specifically binds to the human mNT protein and stabilizes its [2Fe-2S] clusters under oxidative conditions in vitro. Treatment of ovarian cancer cells with NTS-01 induces ovarian cancer (SKOV-3) mitochondrial fragmentation (fission) and reduces ovarian cancer cell proliferation in a 2D single-layer cell culture, as well as in a 3D-spheroids culture. The NTS-01 molecule represents therefore a new lead compound for further drug design studies attempting to develop efficient treatment against ovarian cancer.
LB  - PUB:(DE-HGF)16
C6  - 36374279
UR  - <Go to ISI:>//WOS:000885419200001
DO  - DOI:10.1021/acs.jpcb.2c05728
UR  - https://juser.fz-juelich.de/record/943378
ER  -