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@ARTICLE{Marjault:943378,
author = {Marjault, Henri-Baptiste and Yang-Sung, Sohn and Zuo, Ke
and Carloni, Paolo and Mittler, Ron and Nechushtai, Rachel},
title = {{S}tructure-{B}ased {S}creening {R}eveals a {L}igand {T}hat
{S}tabilizes the [2{F}e-2{S}] {C}lusters of {H}uman
mito{NEET} and {R}educes {O}varian {C}ancer {C}ell
{P}roliferation},
journal = {The journal of physical chemistry / B},
volume = {126},
number = {46},
issn = {1520-6106},
address = {Washington, DC},
publisher = {Soc.},
reportid = {FZJ-2023-00977},
pages = {9559 - 9565},
year = {2022},
abstract = {Human NEET proteins play an important role in a variety of
diseases, including cancer. Using the recently published
X-ray structure of the human mNT-M1 complex, we screened a
commercial chemical compound library and identified a new
human mitoNEET (mNT) binding ligand (NTS-01). Biochemical
investigations revealed that NTS-01 specifically binds to
the human mNT protein and stabilizes its [2Fe-2S] clusters
under oxidative conditions in vitro. Treatment of ovarian
cancer cells with NTS-01 induces ovarian cancer (SKOV-3)
mitochondrial fragmentation (fission) and reduces ovarian
cancer cell proliferation in a 2D single-layer cell culture,
as well as in a 3D-spheroids culture. The NTS-01 molecule
represents therefore a new lead compound for further drug
design studies attempting to develop efficient treatment
against ovarian cancer.},
cin = {IAS-5 / INM-9},
ddc = {530},
cid = {I:(DE-Juel1)IAS-5-20120330 / I:(DE-Juel1)INM-9-20140121},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {36374279},
UT = {WOS:000885419200001},
doi = {10.1021/acs.jpcb.2c05728},
url = {https://juser.fz-juelich.de/record/943378},
}