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@ARTICLE{Hornbergs:972159,
author = {Hornbergs, Jannik and Montag, Karolin and Loschwitz,
Jennifer and Mohr, Inga and Poschmann, Gereon and Schnake,
Anika and Gratz, Regina and Brumbarova, Tzvetina and
Eutebach, Monique and Angrand, Kalina and Fink-Straube,
Claudia and Stühler, Kai and Zeier, Jürgen and Hartmann,
Laura and Strodel, Birgit and Ivanov, Rumen and Bauer,
Petra},
title = {{SEC}14-{GOLD} protein {PATELLIN}2 binds {IRON}-{REGULATED}
{TRANSPORTER}1 linking root iron uptake to vitamin {E}},
journal = {Plant physiology},
volume = {192},
number = {1},
issn = {0032-0889},
address = {Oxford},
publisher = {Oxford University Press},
reportid = {FZJ-2023-01103},
pages = {504–526},
year = {2022},
abstract = {Organisms require micronutrients, and Arabidopsis
(Arabidopsis thaliana) IRON-REGULATED TRANSPORTER1 (IRT1) is
essential for iron (Fe2+) acquisition into root cells.
Uptake of reactive Fe2+ exposes cells to the risk of
membrane lipid peroxidation. Surprisingly little is known
about how this is avoided. IRT1 activity is controlled by an
intracellular variable region (IRT1vr) that acts as a
regulatory protein interaction platform. Here, we describe
that IRT1vr interacted with peripheral plasma membrane
SEC14-Golgi dynamics (SEC14-GOLD) protein PATELLIN2 (PATL2).
SEC14 proteins bind lipophilic substrates and transport or
present them at the membrane. To date, no direct roles have
been attributed to SEC14 proteins in Fe import. PATL2
affected root Fe acquisition responses, interacted with ROS
response proteins in roots, and alleviated root lipid
peroxidation. PATL2 had high affinity in vitro for the major
lipophilic antioxidant vitamin E compound α-tocopherol.
Molecular dynamics simulations provided insight into
energetic constraints and the orientation and stability of
the PATL2-ligand interaction in atomic detail. Hence, this
work highlights a compelling mechanism connecting vitamin E
with root metal ion transport at the plasma membrane with
the participation of an IRT1-interacting and
α-tocopherol-binding SEC14 protein.},
cin = {IBI-7},
ddc = {580},
cid = {I:(DE-Juel1)IBI-7-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {36493393},
UT = {WOS:000927167100001},
doi = {10.1093/plphys/kiac563},
url = {https://juser.fz-juelich.de/record/972159},
}